TY - JOUR
T1 - A high sensitivity bead-based immunoassay with nanofluidic preconcentration for biomarker detection
AU - Fan, Yu Jui
AU - Deng, Chih Zong
AU - Chung, Pei Shan
AU - Tian, Wei Cheng
AU - Sheen, Horn Jiunn
PY - 2018/11/1
Y1 - 2018/11/1
N2 - In this study, we developed a rapid biomarker sensor with a high sensitivity and high selectivity by applying a bead-based immunosensing technique in a nanofluidic preconcentration device. Antibody-coated 300-nm beads and antigens were mixed and then pumped into a valve-integrated nanofluidic preconcentration device for collection and trapping. Because of size differences between the molecules and 300-nm beads, the antigen (molecule) in the mixed solution could be concentrated by up to 10,000-fold in 2 min, but the nanobeads could not be thus concentrated. After concentrated antigens and antibody-coated nanobeads were isolated, micro-particle tracking velocimetry was used to measure the Brownian diffusion of the nanobeads in real time to determine the antigen concentration. Through this preconcentration experiment, we demonstrated a rapid and high-sensitivity detection of prostate-specific antigen with a detection limit of 50 pg/ml in 20 min.
AB - In this study, we developed a rapid biomarker sensor with a high sensitivity and high selectivity by applying a bead-based immunosensing technique in a nanofluidic preconcentration device. Antibody-coated 300-nm beads and antigens were mixed and then pumped into a valve-integrated nanofluidic preconcentration device for collection and trapping. Because of size differences between the molecules and 300-nm beads, the antigen (molecule) in the mixed solution could be concentrated by up to 10,000-fold in 2 min, but the nanobeads could not be thus concentrated. After concentrated antigens and antibody-coated nanobeads were isolated, micro-particle tracking velocimetry was used to measure the Brownian diffusion of the nanobeads in real time to determine the antigen concentration. Through this preconcentration experiment, we demonstrated a rapid and high-sensitivity detection of prostate-specific antigen with a detection limit of 50 pg/ml in 20 min.
KW - Bead-immunoassay
KW - Brownian motion
KW - Micro-PTV
KW - Nanofluidic preconcentrator
UR - http://www.scopus.com/inward/record.url?scp=85048573340&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048573340&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2018.05.141
DO - 10.1016/j.snb.2018.05.141
M3 - Article
AN - SCOPUS:85048573340
VL - 272
SP - 502
EP - 509
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
ER -