A functional polymorphism in the promoter region of TLR3 is associated with susceptibility to end-stage renal disease

Hsin Yi Yang, Shih Ming Huang, Kuo Cheng Lu, Chia Chao Wu, Chi Yu Kang, Yuh Feng Lin, Chin Lin, Fu Huang Lin, Sen Yeong Kao, Sui Lung Su

研究成果: 雜誌貢獻文章

1 引文 斯高帕斯(Scopus)

摘要

Background/Aims: End-stage renal disease (ESRD) is simultaneously associated with immune activation, systemic inflammation and immune deficiency. Toll-like receptor 3 (TLR3), a receptor for viral double-stranded RNA, is involved in immune cell activation in renal diseases and may contribute to chronic inflammatory disease progression. To date, effects of TLR3 polymorphisms on ESRD remain unknown. Therefore, we determined the predictive value of TLR3 polymorphisms and further functionally studied ESRD. Methods: We performed a case-control association study and genotyped 616 ESRD patients and 813 healthy controls. Patients were genotyped for -7C/A, 1377C/T and 1234C/T polymorphisms of TLR3 using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The Haplotype association analysis was performed using the Haploview package. A luciferase reporter assay and real-time PCR were used to test the function of the -7C/A promoter polymorphism in TLR3 expression in human embryonic kidney 293 (HEK293) cells. Results: Genotype distributions of -7C/A and 1377C/T in TLR3 were significantly different in ESRD patients and healthy controls. The ATC haplotype of TLR3 was associated with a decreased risk of ESRD. We also found significant differences in TLR3 expression by dexamethasone treatment between various genotypes of -7C/A (p = 0.02). TLR3 transcriptional activity of the variant -7 C allele was higher than that of the -7 A allele after dexamethasone treatment. Conclusion: Results indicate that, in our population, the presence of the C allele of -7C/A in TLR3 increases the susceptibility to ESRD. In vitro studies demonstrated that -7C/A may be involved in ESRD development through transcriptional modulation of TLR3.

原文英語
頁(從 - 到)131-139
頁數9
期刊American Journal of Nephrology
40
發行號2
DOIs
出版狀態已發佈 - 五月 22 2014

ASJC Scopus subject areas

  • Nephrology
  • Medicine(all)

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    Yang, H. Y., Huang, S. M., Lu, K. C., Wu, C. C., Kang, C. Y., Lin, Y. F., Lin, C., Lin, F. H., Kao, S. Y., & Su, S. L. (2014). A functional polymorphism in the promoter region of TLR3 is associated with susceptibility to end-stage renal disease. American Journal of Nephrology, 40(2), 131-139. https://doi.org/10.1159/000365934