A feasible tool to detect mRNA expression of matrix metalloproteinases and their tissue inhibitors in human Tenon's capsule

Yu Lun Huang, Catherine J. Liu, Allen W. Chiu, Yu Chun Wang, Steven K. Huan, Fenq Lih Lee, Shih Jen Chen, Wen-Ming Hsu, Shie Liang Hsieh

研究成果: 雜誌貢獻文章同行評審

5 引文 斯高帕斯(Scopus)

摘要

Purpose: To determine the mRNA expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in human specimens of Tenon's capsule. Methods: Reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for MMP-1, MMP-2, MMP-9, TIMP-1 and TIMP-2 was performed on tissue specimens obtained from patients with cataract, rhegmatogenous retinal detachment, glaucoma, or diabetes mellitus. Results: Glyceraldehyde phosphate dehydrogenase (GAPDH) mRNA transcripts were detected in 26 (76.5%) of 34 specimens, with almost the same amount of expression in each of these samples. Messenger RNA expression of one or more of the MMPs/TIMPs could also be detected in all of these 26 samples, but not in any sample without GAPDH expression. MMP-2, TIMP-1 and TIMP-2 were detected in 25 (96.2%) of the 26 samples with GAPDH expression, while MMP-1 and MMP-9 were detected with a lower percentage (34.6 and 19.2%, respectively). Conclusions: The feasibility of RT-PCR with GAPDH as an internal standard to determine mRNA transcripts of the MMPs and TIMPs in the subcon-junctival Tenon's capsule was demonstrated.

原文英語
頁(從 - 到)375-379
頁數5
期刊Ophthalmic Research
34
發行號6
DOIs
出版狀態已發佈 - 12月 1 2002
對外發佈

ASJC Scopus subject areas

  • 眼科
  • 感覺系統
  • 細胞與分子神經科學

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