A Decrease in the Percentage of CD3+ Cells Is Correlated With Clinical Improvement During Plasmapheresis in Patients With Myasthenia Gravis

Pei Ju Chien, Jiann Horng Yeh, Chwen Ming Shih, Yu Mei Hsueh, Mei Chieh Chen, Hou Chang Chiu

研究成果: 雜誌貢獻文章

3 引文 (Scopus)

摘要

Plasmapheresis not only removes circulating antibodies but also modulates cellular immunity, including lymphocyte subsets. To investigate the effect of double-filtration plasmapheresis (DFPP) on the ratio of lymphocyte subsets in patients with myasthenia gravis (MG), we examined the percentages of B-cells, T-cells, T helper (Th) cells, T suppressor (Ts) cells, natural killer (NK) cells, NKT cells, and Th/Ts ratio before and after a single DFPP session and after a course of DFPP. A total of 26 patients were recruited; their peripheral blood lymphocyte subsets were assayed using flow cytometry. After a single session of DFPP treatment, the percentages of T-cells (P=0.0200), Th cells (P=0.0178), and the Th/Ts ratio (P=0.0309) decreased significantly, whereas the percentage of NK cells (P=0.0007) increased significantly. More importantly, after one course of DFPP treatment, the reduced clinical quantitative MG (QMG) score was correlated with the decrease of the percentage of T-cells (r=0.5005, P=0.0092). Fourteen thymectomized MG patients had decreased percentages of T-cells (P=0.0304) and Th cells (P=0.0444), whereas they had increased NK cells (P=0.0197) after a single DFPP session. Here, transiently decreased percentages of T-cells after the full DFPP course could enhance the effectiveness of plasmapheresis for MG patients. Artificial Organs
原文英語
頁(從 - 到)211-216
頁數6
期刊Artificial Organs
37
發行號2
DOIs
出版狀態已發佈 - 二月 2013

指紋

Plasmapheresis
Myasthenia Gravis
T-cells
Lymphocytes
Helper-Inducer T-Lymphocytes
T-Lymphocytes
Lymphocyte Subsets
Natural Killer Cells
Artificial organs
Flow cytometry
Artificial Organs
Antibodies
Natural Killer T-Cells
Blood
Cellular Immunity
Cells
Flow Cytometry
B-Lymphocytes

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

引用此文

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title = "A Decrease in the Percentage of CD3+ Cells Is Correlated With Clinical Improvement During Plasmapheresis in Patients With Myasthenia Gravis",
abstract = "Plasmapheresis not only removes circulating antibodies but also modulates cellular immunity, including lymphocyte subsets. To investigate the effect of double-filtration plasmapheresis (DFPP) on the ratio of lymphocyte subsets in patients with myasthenia gravis (MG), we examined the percentages of B-cells, T-cells, T helper (Th) cells, T suppressor (Ts) cells, natural killer (NK) cells, NKT cells, and Th/Ts ratio before and after a single DFPP session and after a course of DFPP. A total of 26 patients were recruited; their peripheral blood lymphocyte subsets were assayed using flow cytometry. After a single session of DFPP treatment, the percentages of T-cells (P=0.0200), Th cells (P=0.0178), and the Th/Ts ratio (P=0.0309) decreased significantly, whereas the percentage of NK cells (P=0.0007) increased significantly. More importantly, after one course of DFPP treatment, the reduced clinical quantitative MG (QMG) score was correlated with the decrease of the percentage of T-cells (r=0.5005, P=0.0092). Fourteen thymectomized MG patients had decreased percentages of T-cells (P=0.0304) and Th cells (P=0.0444), whereas they had increased NK cells (P=0.0197) after a single DFPP session. Here, transiently decreased percentages of T-cells after the full DFPP course could enhance the effectiveness of plasmapheresis for MG patients. Artificial Organs",
keywords = "Lymphocyte subsets, Myasthenia gravis, Plasma exchange, Plasmapheresis, Thymectomy",
author = "Chien, {Pei Ju} and Yeh, {Jiann Horng} and Shih, {Chwen Ming} and Hsueh, {Yu Mei} and Chen, {Mei Chieh} and Chiu, {Hou Chang}",
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T1 - A Decrease in the Percentage of CD3+ Cells Is Correlated With Clinical Improvement During Plasmapheresis in Patients With Myasthenia Gravis

AU - Chien, Pei Ju

AU - Yeh, Jiann Horng

AU - Shih, Chwen Ming

AU - Hsueh, Yu Mei

AU - Chen, Mei Chieh

AU - Chiu, Hou Chang

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N2 - Plasmapheresis not only removes circulating antibodies but also modulates cellular immunity, including lymphocyte subsets. To investigate the effect of double-filtration plasmapheresis (DFPP) on the ratio of lymphocyte subsets in patients with myasthenia gravis (MG), we examined the percentages of B-cells, T-cells, T helper (Th) cells, T suppressor (Ts) cells, natural killer (NK) cells, NKT cells, and Th/Ts ratio before and after a single DFPP session and after a course of DFPP. A total of 26 patients were recruited; their peripheral blood lymphocyte subsets were assayed using flow cytometry. After a single session of DFPP treatment, the percentages of T-cells (P=0.0200), Th cells (P=0.0178), and the Th/Ts ratio (P=0.0309) decreased significantly, whereas the percentage of NK cells (P=0.0007) increased significantly. More importantly, after one course of DFPP treatment, the reduced clinical quantitative MG (QMG) score was correlated with the decrease of the percentage of T-cells (r=0.5005, P=0.0092). Fourteen thymectomized MG patients had decreased percentages of T-cells (P=0.0304) and Th cells (P=0.0444), whereas they had increased NK cells (P=0.0197) after a single DFPP session. Here, transiently decreased percentages of T-cells after the full DFPP course could enhance the effectiveness of plasmapheresis for MG patients. Artificial Organs

AB - Plasmapheresis not only removes circulating antibodies but also modulates cellular immunity, including lymphocyte subsets. To investigate the effect of double-filtration plasmapheresis (DFPP) on the ratio of lymphocyte subsets in patients with myasthenia gravis (MG), we examined the percentages of B-cells, T-cells, T helper (Th) cells, T suppressor (Ts) cells, natural killer (NK) cells, NKT cells, and Th/Ts ratio before and after a single DFPP session and after a course of DFPP. A total of 26 patients were recruited; their peripheral blood lymphocyte subsets were assayed using flow cytometry. After a single session of DFPP treatment, the percentages of T-cells (P=0.0200), Th cells (P=0.0178), and the Th/Ts ratio (P=0.0309) decreased significantly, whereas the percentage of NK cells (P=0.0007) increased significantly. More importantly, after one course of DFPP treatment, the reduced clinical quantitative MG (QMG) score was correlated with the decrease of the percentage of T-cells (r=0.5005, P=0.0092). Fourteen thymectomized MG patients had decreased percentages of T-cells (P=0.0304) and Th cells (P=0.0444), whereas they had increased NK cells (P=0.0197) after a single DFPP session. Here, transiently decreased percentages of T-cells after the full DFPP course could enhance the effectiveness of plasmapheresis for MG patients. Artificial Organs

KW - Lymphocyte subsets

KW - Myasthenia gravis

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