A constitutively mannose-sensitive agglutinating Salmonella enterica subsp. enterica serovar Typhimurium strain, carrying a transposon in the fimbrial usher gene stbC, exhibits multidrug resistance and flagellated phenotypes

Kuan Hsun Wu, Ke Chuan Wang, Lin Wen Lee, Yi Ning Huang, Kuang-Sheng Yeh

研究成果: 雜誌貢獻文章

6 引文 (Scopus)

摘要

Static broth culture favors Salmonella enterica subsp. enterica serovar Typhimurium to produce type 1 fimbriae, while solid agar inhibits its expression. A transposon inserted in stbC, which would encode an usher for Stb fimbriae of a non-flagellar Salmonella enterica subsp. enterica serovar Typhimurium LB5010 strain, conferred it to agglutinate yeast cells on both cultures. RT-PCR revealed that the expression of the fimbrial subunit gene fimA, and fimZ, a regulatory gene of fimA, were both increased in the stbC mutant when grown on LB agar; fimW, a repressor gene of fimA, exhibited lower expression. Flagella were observed in the stbC mutant and this phenotype was correlated with the motile phenotype. Microarray data and RT-PCR indicated that the expression of three genes, motA, motB, and cheM, was enhanced in the stbC mutant. The stbC mutant was resistant to several antibiotics, consistent with the finding that expression of yhcQ and ramA was enhanced. A complementation test revealed that transforming a recombinant plasmid possessing the stbC restored the mannose-sensitive agglutination phenotype to the stbC mutant much as that in the parental Salmonella enterica subsp. enterica serovar Typhimurium LB5010 strain, indicating the possibility of an interplay of different fimbrial systems in coordinating their expression.
原文英語
文章編號280264
期刊Scientific World Journal
2012
DOIs
出版狀態已發佈 - 2012

指紋

Salmonella
Salmonella enterica
Multiple Drug Resistance
Mannose
phenotype
Genes
Phenotype
Agar
gene
Genetic Complementation Test
Polymerase Chain Reaction
Flagella
agar
Agglutination
Regulator Genes
Plasmids
Microarrays
Yeasts
Cell culture
Yeast

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

引用此文

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title = "A constitutively mannose-sensitive agglutinating Salmonella enterica subsp. enterica serovar Typhimurium strain, carrying a transposon in the fimbrial usher gene stbC, exhibits multidrug resistance and flagellated phenotypes",
abstract = "Static broth culture favors Salmonella enterica subsp. enterica serovar Typhimurium to produce type 1 fimbriae, while solid agar inhibits its expression. A transposon inserted in stbC, which would encode an usher for Stb fimbriae of a non-flagellar Salmonella enterica subsp. enterica serovar Typhimurium LB5010 strain, conferred it to agglutinate yeast cells on both cultures. RT-PCR revealed that the expression of the fimbrial subunit gene fimA, and fimZ, a regulatory gene of fimA, were both increased in the stbC mutant when grown on LB agar; fimW, a repressor gene of fimA, exhibited lower expression. Flagella were observed in the stbC mutant and this phenotype was correlated with the motile phenotype. Microarray data and RT-PCR indicated that the expression of three genes, motA, motB, and cheM, was enhanced in the stbC mutant. The stbC mutant was resistant to several antibiotics, consistent with the finding that expression of yhcQ and ramA was enhanced. A complementation test revealed that transforming a recombinant plasmid possessing the stbC restored the mannose-sensitive agglutination phenotype to the stbC mutant much as that in the parental Salmonella enterica subsp. enterica serovar Typhimurium LB5010 strain, indicating the possibility of an interplay of different fimbrial systems in coordinating their expression.",
author = "Wu, {Kuan Hsun} and Wang, {Ke Chuan} and Lee, {Lin Wen} and Huang, {Yi Ning} and Kuang-Sheng Yeh",
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T1 - A constitutively mannose-sensitive agglutinating Salmonella enterica subsp. enterica serovar Typhimurium strain, carrying a transposon in the fimbrial usher gene stbC, exhibits multidrug resistance and flagellated phenotypes

AU - Wu, Kuan Hsun

AU - Wang, Ke Chuan

AU - Lee, Lin Wen

AU - Huang, Yi Ning

AU - Yeh, Kuang-Sheng

PY - 2012

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