A chemically functionalized magnetic nanoplatform for rapid and specific biomolecular recognition and separation

Po Chiao Lin, Ching Ching Yu, Huan Ting Wu, Ying Wei Lu, Chia Li Han, An Kai Su, Yu Ju Chen, Chun Cheng Lin

研究成果: 雜誌貢獻文章同行評審

26 引文 斯高帕斯(Scopus)

摘要

We have developed a target-molecule-functionalized magnetic nanoparticle (MNP)-based method to facilitate the study of biomolecular recognition and separation. The superparamagnetic property of MNPs allows the corresponding biomolecules to be rapidly separated from crude biofluids with a significant improvement in recovery yield and specificity. Various MNPs functionalized with tag molecules (chitin, heparin, and amylose) were synthesized for recombinant protein purification, and several probe-functionalized MNPs, such as nitrilotriacetic acid (NTA)@MNP and Pk@MNP, exhibited excellent extraction efficiency for proteins. In a cell recognition study, mannose-functionalized MNPs allowed specific purification of Escherichia coli with FimH adhesin on the surface. In an immunoprecipitation assay, the antibody-conjugated MNPs reduced the incubation time from 12 to 1 h while maintaining a comparable efficiency. The functionalized MNPs were also used in a membrane proteomic study that utilized the interaction between streptavidin-functionalized MNPs and biotinylated cell membrane proteins. Overall, the functionalized MNPs were demonstrated to be promising probes for the specific separation of targets from proteins to cells and proteomics.

原文英語
頁(從 - 到)160-168
頁數9
期刊Biomacromolecules
14
發行號1
DOIs
出版狀態已發佈 - 一月 14 2013
對外發佈Yes

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials

指紋 深入研究「A chemically functionalized magnetic nanoplatform for rapid and specific biomolecular recognition and separation」主題。共同形成了獨特的指紋。

引用此