A broad range fluorescent pH sensor based on hollow mesoporous silica nanoparticles, utilising the surface curvature effect

Chieh Jui Tsou, Chia Yin Chu, Yann Hung, Chung Yuan Mou

研究成果: 雜誌貢獻文章同行評審

43 引文 斯高帕斯(Scopus)

摘要

In this study, a broad range pH sensor was synthesized by loading the pH sensitive dye, fluorescein isothiocyanate (FITC), and a reference dye, rhodamine B isothiocyanate (RITC), into the mesostructure of hollow mesoporous silica nanoparticles (HMSNs) synthesized using a co-condensation method. Compared to a pH sensor based on the same pair of dyes on conventional mesoporous silica nanoparticles (MSNs), this dual-labeled pH sensor based on HMSNs shows a larger pH sensitive range, between 4.5 and 8.5, because of its broad surface curvature distribution which has a strong effect on the apparent pKa values of the FITC dye. The hollow mesoporous silica-dye nanoparticles were used to monitor intracellular pH via a ratiometric fluorescence method. The confocal images demonstrated the capacity of this broad range sensor which can differentiate simultaneously the local pH between various environments, for example, in the medium (pH = 7.2), cytosol (pH ∼ 7) and the endosome-lysosome pathway (pH = 4-5.5).

原文英語
頁(從 - 到)5557-5563
頁數7
期刊Journal of Materials Chemistry B
1
發行號41
DOIs
出版狀態已發佈 - 11月 7 2013
對外發佈

ASJC Scopus subject areas

  • 化學 (全部)
  • 生物醫學工程
  • 材料科學(全部)

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