摘要
We previously reported that 9-cis retinoic acid (RA) treatment induced an increase in mitochondrial (mt)DNA transcription. In order to extend these results, we tested various concentrations of 9-cis RA were used to treat 143B cells. Cells with low membrane potential treated with 9-cis RA showed significantly lower amounts of RXRα in mitochondria. We also found lower RXRα levels in mtDNA-depleted cells. Treating cells with 9-cis RA significantly increased expression of ND1, ND6, and COX I RNA. However, 9-cis RA-treatment did not appear to induce any significant changes in mtDNA copy number or mitochondrial mass. This study represents that 9-cis RA increases mtDNA transcription but not mtDNA replication, and it suggests that the effects of 9-cis RA on mitochondria are mediated by RXR localization to mitochondria. In addition, this is the first report that 9-cis RA regulation of RXR mitochondrial translocation depends on mitochondrial membrane potential and ATP.
原文 | 英語 |
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頁(從 - 到) | 351-354 |
頁數 | 4 |
期刊 | Biochemical and Biophysical Research Communications |
卷 | 377 |
發行號 | 2 |
DOIs | |
出版狀態 | 已發佈 - 12月 12 2008 |
ASJC Scopus subject areas
- 生物化學
- 生物物理學
- 細胞生物學
- 分子生物學