We previously reported that 9-cis retinoic acid (RA) treatment induced an increase in mitochondrial (mt)DNA transcription. In order to extend these results, we tested various concentrations of 9-cis RA were used to treat 143B cells. Cells with low membrane potential treated with 9-cis RA showed significantly lower amounts of RXRα in mitochondria. We also found lower RXRα levels in mtDNA-depleted cells. Treating cells with 9-cis RA significantly increased expression of ND1, ND6, and COX I RNA. However, 9-cis RA-treatment did not appear to induce any significant changes in mtDNA copy number or mitochondrial mass. This study represents that 9-cis RA increases mtDNA transcription but not mtDNA replication, and it suggests that the effects of 9-cis RA on mitochondria are mediated by RXR localization to mitochondria. In addition, this is the first report that 9-cis RA regulation of RXR mitochondrial translocation depends on mitochondrial membrane potential and ATP.
|頁（從 - 到）||351-354|
|期刊||Biochemical and Biophysical Research Communications|
|出版狀態||已發佈 - 十二月 12 2008|
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Lin, Y. W., Lien, L. M., Yeh, T. S., Wu, H. M., Liu, Y. L., & Hsieh, R. H. (2008). 9-cis retinoic acid induces retinoid X receptor localized to the mitochondria for mediation of mitochondrial transcription. Biochemical and Biophysical Research Communications, 377(2), 351-354. https://doi.org/10.1016/j.bbrc.2008.09.122