2.6 Å X-ray crystal structure of human p53R2, a p53-inducible ribonucleotide reductase

Peter Smith, Bingsen Zhou, Nam Ho, Yate Ching Yuan, Leila Su, Shiou Chuan Tsai, Yun Yen

研究成果: 雜誌貢獻文章

28 引文 (Scopus)

摘要

Human p53R2 (hp53R2) is a 351-residue p53-inducible ribonucleotide reductase (RNR) small subunit. It shares >80% sequence identity with hRRM2, the small RNR subunit responsible for normal maintenance of the deoxyribonucleotide (dNTP) pool used for DNA replication, which is active during the S phase in a cell cycle-dependent fashion. But rather than cyclic dNTP synthesis, hp53R2 has been shown to supply dNTPs forDNA repair to cells in G0-G1 in a p53-dependent fashion. The first X-ray crystal structure of hp53R2 is determined to 2.6 Å, in which monomers A and B exhibit mono- and binuclear iron occupancy, respectively. The pronounced structural differences at three regions between hp53R2 and hRRM2 highlight the possible regulatory role in iron assimilation and help explain previously observed physical and biochemical differences in the mobility and accessibility of the radical iron center, as well as radical transfer pathways between the two enzymes. The sequence-structure-function correlations that differentiate hp53R2 and hRRM2 are revealed for the first time. Insight gained fromthis structural work will be used in the identification of biological function, regulation mechanism, and inhibitor selection in RNR small subunits.

原文英語
頁(從 - 到)11134-11141
頁數8
期刊Biochemistry
48
發行號46
DOIs
出版狀態已發佈 - 十一月 24 2009
對外發佈Yes

指紋

Ribonucleotide Reductases
Iron
Crystal structure
X-Rays
X rays
Deoxyribonucleotides
Repair
Monomers
Cells
DNA Replication
S Phase
DNA
Enzymes
Cell Cycle
Maintenance

ASJC Scopus subject areas

  • Biochemistry

引用此文

2.6 Å X-ray crystal structure of human p53R2, a p53-inducible ribonucleotide reductase. / Smith, Peter; Zhou, Bingsen; Ho, Nam; Yuan, Yate Ching; Su, Leila; Tsai, Shiou Chuan; Yen, Yun.

於: Biochemistry, 卷 48, 編號 46, 24.11.2009, p. 11134-11141.

研究成果: 雜誌貢獻文章

Smith, P, Zhou, B, Ho, N, Yuan, YC, Su, L, Tsai, SC & Yen, Y 2009, '2.6 Å X-ray crystal structure of human p53R2, a p53-inducible ribonucleotide reductase', Biochemistry, 卷 48, 編號 46, 頁 11134-11141. https://doi.org/10.1021/bi9001425
Smith P, Zhou B, Ho N, Yuan YC, Su L, Tsai SC 等. 2.6 Å X-ray crystal structure of human p53R2, a p53-inducible ribonucleotide reductase. Biochemistry. 2009 11月 24;48(46):11134-11141. https://doi.org/10.1021/bi9001425
Smith, Peter ; Zhou, Bingsen ; Ho, Nam ; Yuan, Yate Ching ; Su, Leila ; Tsai, Shiou Chuan ; Yen, Yun. / 2.6 Å X-ray crystal structure of human p53R2, a p53-inducible ribonucleotide reductase. 於: Biochemistry. 2009 ; 卷 48, 編號 46. 頁 11134-11141.
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