2,2′-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls

Ching Chuan Kuo, Wenchang Chiang, Guey Ping Liu, Ya Lin Chien, Jang Yang Chang, Ching Kuo Lee, Jir Mehng Lo, Shou Ling Huang, Ming Chih Shih, Yueh Hsiung Kuo

研究成果: 雜誌貢獻文章

68 引文 (Scopus)

摘要

An activity-directed fractionation and purification process was used to identify the antioxidative components of adlay hulls. Hulls of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) were extracted with methanol and then separated into water, 1-butanol, ethyl acetate, and hexane fractions. The 1-butanol-soluble fraction exhibited greater capacity to scavenge 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radicals when compared with fractions soluble in water, ethyl acetate, and hexane phases. The 1-butanol fraction was then subjected to separation and purification using Diaion HP-20 chromatography, silica gel chromatography, and HPLC. Six compounds showing strong antioxidant activity were identified by spectroscopic methods (1H NMR, 13C NMR, IR, and MS) and by comparison with authentic samples to be coniferyl alcohol (1), syringic acid (2), ferulic acid (3), syringaresinol (4), 4-ketopinoresinol (5), and a new lignan, mayuenolide (6).

原文英語
頁(從 - 到)5850-5855
頁數6
期刊Journal of Agricultural and Food Chemistry
50
發行號21
DOIs
出版狀態已發佈 - 十月 9 2002
對外發佈Yes

指紋

Coix
Coix lacryma-jobi
1-Butanol
Scavenging
butanol
hulls
ferulic acid
Hexanes
Chromatography
hexane
Purification
Nuclear magnetic resonance
coniferyl alcohol
syringic acid
Lignans
purification methods
Water
Silica Gel
lignans
silica gel

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

引用此文

2,2′-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls. / Kuo, Ching Chuan; Chiang, Wenchang; Liu, Guey Ping; Chien, Ya Lin; Chang, Jang Yang; Lee, Ching Kuo; Lo, Jir Mehng; Huang, Shou Ling; Shih, Ming Chih; Kuo, Yueh Hsiung.

於: Journal of Agricultural and Food Chemistry, 卷 50, 編號 21, 09.10.2002, p. 5850-5855.

研究成果: 雜誌貢獻文章

Kuo, Ching Chuan ; Chiang, Wenchang ; Liu, Guey Ping ; Chien, Ya Lin ; Chang, Jang Yang ; Lee, Ching Kuo ; Lo, Jir Mehng ; Huang, Shou Ling ; Shih, Ming Chih ; Kuo, Yueh Hsiung. / 2,2′-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls. 於: Journal of Agricultural and Food Chemistry. 2002 ; 卷 50, 編號 21. 頁 5850-5855.
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abstract = "An activity-directed fractionation and purification process was used to identify the antioxidative components of adlay hulls. Hulls of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) were extracted with methanol and then separated into water, 1-butanol, ethyl acetate, and hexane fractions. The 1-butanol-soluble fraction exhibited greater capacity to scavenge 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radicals when compared with fractions soluble in water, ethyl acetate, and hexane phases. The 1-butanol fraction was then subjected to separation and purification using Diaion HP-20 chromatography, silica gel chromatography, and HPLC. Six compounds showing strong antioxidant activity were identified by spectroscopic methods (1H NMR, 13C NMR, IR, and MS) and by comparison with authentic samples to be coniferyl alcohol (1), syringic acid (2), ferulic acid (3), syringaresinol (4), 4-ketopinoresinol (5), and a new lignan, mayuenolide (6).",
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AU - Liu, Guey Ping

AU - Chien, Ya Lin

AU - Chang, Jang Yang

AU - Lee, Ching Kuo

AU - Lo, Jir Mehng

AU - Huang, Shou Ling

AU - Shih, Ming Chih

AU - Kuo, Yueh Hsiung

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