11H-Isoquino[4,3-c]cinnolin-12-ones: Novel anticancer agents with potent topoisomerase I-targeting activity and cytotoxicity

Alexander L. Ruchelman, Sudhir K. Singh, Abhijit Ray, Xiaohua Wu, Jin Ming Yang, Nai Zhou, Angela Liu, Leroy-Fong Liu, Edmond J. LaVoie

研究成果: 雜誌貢獻文章

48 引文 (Scopus)

摘要

Recent studies have identified 2,3-dimethoxy-8,9-methylenedioxy-11-[(2- dimethylamino)ethyl]-11H-isoquino[4,3-c]cinnolin-12-one (1a) as a novel topoisomerase I-targeting agent with potent cytotoxic activity. The effect of varied substituents at the 11-position of 2,3-dimethoxy-8,9-methylenedioxy-11H- isoquino[4,3-c]cinnolin-12-ones on topoisomerase I-targeting activity and cytotoxicity was evaluated. Potent TOP1-targeting activity was observed when the 11-position was substituted with either a 2-(N,N-dimethylamino)ethyl, a 2-(N,N-diethylamino)ethyl, a n-butyl, or a 2-(pyrrolidin-1-yl)ethyl group. The addition of a β-methyl group to 1a provided an analogue with dramatically reduced TOP1-targeting activity and cytotoxicity. Analogues of 1a wherein the 2-(N,N-dimethylamino)ethyl group was replaced with a (2-tetrahydrofuranyl) methyl, a 2-(piperidin-1-yl)ethyl, or a 2-(4-methylpiperazin-1-yl)ethyl substituent exhibited decreased activity as TOP1-targeting agents. Replacement of the dimethoxy groups of 1a with hydrogen atoms resulted in an analogue with significantly decreased TOP1-targeting activity and cytotoxicity. Removal of both the vicinal dimethoxyl groups and the methylenedioxy moiety resulted in a complete loss of TOP1-targeting activity. The presence of a 9-nitro substituent in place of the 8,9-methylenedioxy group of 1a resulted in a decrease in relative TOP1-targeting activity and cytotoxicity. Compounds 1a and the 11-n-butyl analogue 1d were evaluated for antitumor activity in the human tumor xenograft model using athymic nude mice. The non-estrogen responsive breast tumor cell line MDA-MB-435 was used in these assays. At dose levels that approached its maximum tolerated dose, 1a proved to be effective in inhibiting tumor growth in vivo when administered orally or by ip injection.

原文英語
頁(從 - 到)795-806
頁數12
期刊Bioorganic and Medicinal Chemistry
12
發行號4
DOIs
出版狀態已發佈 - 二月 15 2004
對外發佈Yes

指紋

Type I DNA Topoisomerase
Cytotoxicity
Nude Mice
Antineoplastic Agents
Tumors
Maximum Tolerated Dose
Tumor Cell Line
Heterografts
Human Activities
Hydrogen
Neoplasms
Breast Neoplasms
Injections
Growth
Assays
Cells
Atoms

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

引用此文

11H-Isoquino[4,3-c]cinnolin-12-ones : Novel anticancer agents with potent topoisomerase I-targeting activity and cytotoxicity. / Ruchelman, Alexander L.; Singh, Sudhir K.; Ray, Abhijit; Wu, Xiaohua; Yang, Jin Ming; Zhou, Nai; Liu, Angela; Liu, Leroy-Fong; LaVoie, Edmond J.

於: Bioorganic and Medicinal Chemistry, 卷 12, 編號 4, 15.02.2004, p. 795-806.

研究成果: 雜誌貢獻文章

Ruchelman, Alexander L. ; Singh, Sudhir K. ; Ray, Abhijit ; Wu, Xiaohua ; Yang, Jin Ming ; Zhou, Nai ; Liu, Angela ; Liu, Leroy-Fong ; LaVoie, Edmond J. / 11H-Isoquino[4,3-c]cinnolin-12-ones : Novel anticancer agents with potent topoisomerase I-targeting activity and cytotoxicity. 於: Bioorganic and Medicinal Chemistry. 2004 ; 卷 12, 編號 4. 頁 795-806.
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abstract = "Recent studies have identified 2,3-dimethoxy-8,9-methylenedioxy-11-[(2- dimethylamino)ethyl]-11H-isoquino[4,3-c]cinnolin-12-one (1a) as a novel topoisomerase I-targeting agent with potent cytotoxic activity. The effect of varied substituents at the 11-position of 2,3-dimethoxy-8,9-methylenedioxy-11H- isoquino[4,3-c]cinnolin-12-ones on topoisomerase I-targeting activity and cytotoxicity was evaluated. Potent TOP1-targeting activity was observed when the 11-position was substituted with either a 2-(N,N-dimethylamino)ethyl, a 2-(N,N-diethylamino)ethyl, a n-butyl, or a 2-(pyrrolidin-1-yl)ethyl group. The addition of a β-methyl group to 1a provided an analogue with dramatically reduced TOP1-targeting activity and cytotoxicity. Analogues of 1a wherein the 2-(N,N-dimethylamino)ethyl group was replaced with a (2-tetrahydrofuranyl) methyl, a 2-(piperidin-1-yl)ethyl, or a 2-(4-methylpiperazin-1-yl)ethyl substituent exhibited decreased activity as TOP1-targeting agents. Replacement of the dimethoxy groups of 1a with hydrogen atoms resulted in an analogue with significantly decreased TOP1-targeting activity and cytotoxicity. Removal of both the vicinal dimethoxyl groups and the methylenedioxy moiety resulted in a complete loss of TOP1-targeting activity. The presence of a 9-nitro substituent in place of the 8,9-methylenedioxy group of 1a resulted in a decrease in relative TOP1-targeting activity and cytotoxicity. Compounds 1a and the 11-n-butyl analogue 1d were evaluated for antitumor activity in the human tumor xenograft model using athymic nude mice. The non-estrogen responsive breast tumor cell line MDA-MB-435 was used in these assays. At dose levels that approached its maximum tolerated dose, 1a proved to be effective in inhibiting tumor growth in vivo when administered orally or by ip injection.",
author = "Ruchelman, {Alexander L.} and Singh, {Sudhir K.} and Abhijit Ray and Xiaohua Wu and Yang, {Jin Ming} and Nai Zhou and Angela Liu and Leroy-Fong Liu and LaVoie, {Edmond J.}",
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T2 - Novel anticancer agents with potent topoisomerase I-targeting activity and cytotoxicity

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AU - Singh, Sudhir K.

AU - Ray, Abhijit

AU - Wu, Xiaohua

AU - Yang, Jin Ming

AU - Zhou, Nai

AU - Liu, Angela

AU - Liu, Leroy-Fong

AU - LaVoie, Edmond J.

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N2 - Recent studies have identified 2,3-dimethoxy-8,9-methylenedioxy-11-[(2- dimethylamino)ethyl]-11H-isoquino[4,3-c]cinnolin-12-one (1a) as a novel topoisomerase I-targeting agent with potent cytotoxic activity. The effect of varied substituents at the 11-position of 2,3-dimethoxy-8,9-methylenedioxy-11H- isoquino[4,3-c]cinnolin-12-ones on topoisomerase I-targeting activity and cytotoxicity was evaluated. Potent TOP1-targeting activity was observed when the 11-position was substituted with either a 2-(N,N-dimethylamino)ethyl, a 2-(N,N-diethylamino)ethyl, a n-butyl, or a 2-(pyrrolidin-1-yl)ethyl group. The addition of a β-methyl group to 1a provided an analogue with dramatically reduced TOP1-targeting activity and cytotoxicity. Analogues of 1a wherein the 2-(N,N-dimethylamino)ethyl group was replaced with a (2-tetrahydrofuranyl) methyl, a 2-(piperidin-1-yl)ethyl, or a 2-(4-methylpiperazin-1-yl)ethyl substituent exhibited decreased activity as TOP1-targeting agents. Replacement of the dimethoxy groups of 1a with hydrogen atoms resulted in an analogue with significantly decreased TOP1-targeting activity and cytotoxicity. Removal of both the vicinal dimethoxyl groups and the methylenedioxy moiety resulted in a complete loss of TOP1-targeting activity. The presence of a 9-nitro substituent in place of the 8,9-methylenedioxy group of 1a resulted in a decrease in relative TOP1-targeting activity and cytotoxicity. Compounds 1a and the 11-n-butyl analogue 1d were evaluated for antitumor activity in the human tumor xenograft model using athymic nude mice. The non-estrogen responsive breast tumor cell line MDA-MB-435 was used in these assays. At dose levels that approached its maximum tolerated dose, 1a proved to be effective in inhibiting tumor growth in vivo when administered orally or by ip injection.

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