κ-Carrageenan Gel Modified Mesoporous Gold Chronocoulometric Sensor for Ultrasensitive Detection of MicroRNA

Bidita Salahuddin, Mostafa Kamal Masud, Shazed Aziz, Chia Hung Liu, Nasim Amiralian, Aditya Ashok, S. M.Azad Hossain, Hyeongyu Park, Md Abdul Wahab, Mohammed A. Amin, M. Adharvana Chari, Alan E. Rowan, Yusuke Yamauchi, Md Shahriar A. Hossain, Yusuf Valentino Kaneti

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)

摘要

Hydrogel-functionalized surface-based transducers demonstrate fluid-like kinetics, non-fouling properties, and superior biocompatibility. The integration of such properties of threedimensional (3D) micro- or macrostructure of hydrogels into a mesoporous platform provides a favorable moiety for incorporating biomolecule for adsorption or hybridization with a capture agent. Herein, we report a novel κ-carrageenan hydrogelcoated mesoporous gold (Au) electrode (abbreviated as MPGE/ gel) for chronocoulometric (CC) detection of microRNA (miRNA). The κ-carrageenan gel provides a 3D porous network on Au electrode surface to enable higher adsorption of target miRNA for CC interrogation of miRNA in presence of a redox molecule-ruthenium hexaammine (III) chloride ([Ru(NH3)6]3+, RuHex). Magnetically isolated and purified target miR-9-2 is adsorbed onto the MPGE via Au-RNA affinity interaction through the porous 3D network of the gel followed by the CC detection. The enhanced miRNA adsorption and electrocatalytic activity of MPGE/gel provide attomolar (50 aM) level of detection of miRNA with a dynamic range from 100pM to 10 aM. The good reproducibility (% RSD ≤ 5%, for n = 3) and high specificity of the developed biosensor demonstrates its excellent translational potential toward developing precisely controlled sensing devices for current clinical needs.
原文英語
頁(從 - 到)198-207
頁數10
期刊Bulletin of the Chemical Society of Japan
95
發行號1
DOIs
出版狀態已發佈 - 2022

ASJC Scopus subject areas

  • 化學 (全部)

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