β-Sitosterol inhibits cell cycle progression of rat aortic smooth muscle cells through increases of p21cip1 protein

Ming Hsien Chien, Tong Sheng Lee, Yu Chih Liang, Wen Sen Lee

研究成果: 雜誌貢獻 › 文章

5 引文 (Scopus)

摘要

Abnormal proliferation of vascular smooth muscle cells (VSMCs) plays a central role in the pathogenesis of atherosclerosis. β-Sitosterol, an important phytosterol found in plant food, is known to exert antiatherosclerosis activity. However, the molecular mechanisms underlying β-sitosterol- induced antiproliferation of VSMCs were still not clear. This study demonstrated that β-sitosterol (1-20 μM) concentration-dependently inhibited proliferation of rat aortic smooth muscle cells (RASMCs) without cytotoxic effect. Flow cytometric analysis revealed that β-sitosterol arrested cell cycle progression through down-regulation of cyclin E and cyclin-dependent kinase (CDK)2 and up-regulation of p21cip1. In the β-sitosterol-treated RASMCs, the formation of the CDK2-p21cip1 complex was increased and the assayable CDK2 activity was decreased. Knockdown of the expression of p21cip1 gene prevented β-sitosterol-induced cell cycle arrest in RASMCs. In conclusion, β-sitosterol inhibited VSMC proliferation by increasing the levels of p21cip1 protein, which in turn inhibited the CDK2 activity, and finally interrupted the progress of the cell cycle.
原文英語
頁（從 - 到）10064-10069
頁數6
期刊Journal of Agricultural and Food Chemistry
卷58
發行號18
DOIs
出版狀態已發佈 - 九月 22 2010

指紋

sitosterols
smooth muscle
myocytes
Smooth Muscle Myocytes
Muscle
Rats
cell cycle
Cell Cycle
Cells
rats
Vascular Smooth Muscle
blood vessels
Proteins
proteins
Cyclin-Dependent Kinase 2
Phytosterols
Cyclin E
Edible Plants
cyclin-dependent kinase
phytosterols

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

引用此文

β-Sitosterol inhibits cell cycle progression of rat aortic smooth muscle cells through increases of p21cip1 protein. / Chien, Ming Hsien; Lee, Tong Sheng; Liang, Yu Chih; Lee, Wen Sen.

於: Journal of Agricultural and Food Chemistry, 卷 58, 編號 18, 22.09.2010, p. 10064-10069.

研究成果: 雜誌貢獻 › 文章

Chien, MH, Lee, TS, Liang, YC & Lee, WS 2010, 'β-Sitosterol inhibits cell cycle progression of rat aortic smooth muscle cells through increases of p21cip1 protein', Journal of Agricultural and Food Chemistry, 卷 58, 編號 18, 頁 10064-10069. https://doi.org/10.1021/jf102741y
Chien MH, Lee TS, Liang YC, Lee WS. β-Sitosterol inhibits cell cycle progression of rat aortic smooth muscle cells through increases of p21cip1 protein. Journal of Agricultural and Food Chemistry. 2010 9月 22;58(18):10064-10069. https://doi.org/10.1021/jf102741y
Chien, Ming Hsien ; Lee, Tong Sheng ; Liang, Yu Chih ; Lee, Wen Sen. / β-Sitosterol inhibits cell cycle progression of rat aortic smooth muscle cells through increases of p21cip1 protein. 於: Journal of Agricultural and Food Chemistry. 2010 ; 卷 58, 編號 18. 頁 10064-10069.
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