Zwitterionic poly(sulfobetaine methacrylate) hydrogels incorporated with angiogenic peptides promote differentiation of human adipose-derived stem cells

Chung Wei Kao, Po Hsiu Cheng, Po Ting Wu, Shih Wen Wang, I. Chun Chen, Nai Chen Cheng, Kai Chiang Yang, Jiashing Yu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The superhydrophilic and ultralow biofouling properties as well as the resistance to foreign-body reaction make zwitterionic polymer promising in biomedical applications. Incorporation of arginine-glycine-aspartate (RGD) sequence and/or vascular endothelial growth factor mimicking (QK) peptide to zwitterionic poly(sulfobetaine methacrylate) (PSBMA) hydrogels may not only improve cell adhesion but also influence stem cell differentiation. In this study, PSBMA hydrogels were modified with RGD or RGD/QK peptides and characterized. Multipotent human adipose-tissue derived stem cells (hASCs) were seeded to peptides-grafted PSBMA hydrogels, and the cell adhesion, proliferentiation, and differentiation of hASCs on hydrogels were demonstrated. The results showed that grafting of RGD or RGD/QK peptides improved hASCs adhesion, while the mechanical strength of peptides-grafted PSBMA hydrogels was decreased. Incorporation of RGD or RGD/QK peptides did not influence the adsorption adsorption behaviors of PSBMA hydrogels. Relative to RGD-grafted PSBMA hydrogels, the RGD/QK incorporation further improved hASCs differentiated into adipogenic lineage, while RGD or RGD/QK additions had no substantial effects on osteogenesis under osteogenic induction. Moreover, the endothelial differentiation was also enhanced when hASCs cultured on RGD/QK-modified PSBMA hydrogels. In conclusion, the graftings of RGD and RGD/QK did not interfere the nonfouling property of PSBMA hydrogels. The incorporation of RGD/QK peptides can improve hASCs differentiate toward adipogenic/endothelial lineages.

Original languageEnglish
Pages (from-to)51343-51351
Number of pages9
JournalRSC Advances
Volume7
Issue number81
DOIs
Publication statusPublished - Jan 1 2017

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Angiogenic Proteins
Hydrogels
Stem cells
Peptides
Tissue
Cell adhesion
sulfobetaine methacrylate polymer
Biofouling
Adsorption
Arginine

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Zwitterionic poly(sulfobetaine methacrylate) hydrogels incorporated with angiogenic peptides promote differentiation of human adipose-derived stem cells. / Kao, Chung Wei; Cheng, Po Hsiu; Wu, Po Ting; Wang, Shih Wen; Chen, I. Chun; Cheng, Nai Chen; Yang, Kai Chiang; Yu, Jiashing.

In: RSC Advances, Vol. 7, No. 81, 01.01.2017, p. 51343-51351.

Research output: Contribution to journalArticle

Kao, Chung Wei ; Cheng, Po Hsiu ; Wu, Po Ting ; Wang, Shih Wen ; Chen, I. Chun ; Cheng, Nai Chen ; Yang, Kai Chiang ; Yu, Jiashing. / Zwitterionic poly(sulfobetaine methacrylate) hydrogels incorporated with angiogenic peptides promote differentiation of human adipose-derived stem cells. In: RSC Advances. 2017 ; Vol. 7, No. 81. pp. 51343-51351.
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abstract = "The superhydrophilic and ultralow biofouling properties as well as the resistance to foreign-body reaction make zwitterionic polymer promising in biomedical applications. Incorporation of arginine-glycine-aspartate (RGD) sequence and/or vascular endothelial growth factor mimicking (QK) peptide to zwitterionic poly(sulfobetaine methacrylate) (PSBMA) hydrogels may not only improve cell adhesion but also influence stem cell differentiation. In this study, PSBMA hydrogels were modified with RGD or RGD/QK peptides and characterized. Multipotent human adipose-tissue derived stem cells (hASCs) were seeded to peptides-grafted PSBMA hydrogels, and the cell adhesion, proliferentiation, and differentiation of hASCs on hydrogels were demonstrated. The results showed that grafting of RGD or RGD/QK peptides improved hASCs adhesion, while the mechanical strength of peptides-grafted PSBMA hydrogels was decreased. Incorporation of RGD or RGD/QK peptides did not influence the adsorption adsorption behaviors of PSBMA hydrogels. Relative to RGD-grafted PSBMA hydrogels, the RGD/QK incorporation further improved hASCs differentiated into adipogenic lineage, while RGD or RGD/QK additions had no substantial effects on osteogenesis under osteogenic induction. Moreover, the endothelial differentiation was also enhanced when hASCs cultured on RGD/QK-modified PSBMA hydrogels. In conclusion, the graftings of RGD and RGD/QK did not interfere the nonfouling property of PSBMA hydrogels. The incorporation of RGD/QK peptides can improve hASCs differentiate toward adipogenic/endothelial lineages.",
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AU - Kao, Chung Wei

AU - Cheng, Po Hsiu

AU - Wu, Po Ting

AU - Wang, Shih Wen

AU - Chen, I. Chun

AU - Cheng, Nai Chen

AU - Yang, Kai Chiang

AU - Yu, Jiashing

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N2 - The superhydrophilic and ultralow biofouling properties as well as the resistance to foreign-body reaction make zwitterionic polymer promising in biomedical applications. Incorporation of arginine-glycine-aspartate (RGD) sequence and/or vascular endothelial growth factor mimicking (QK) peptide to zwitterionic poly(sulfobetaine methacrylate) (PSBMA) hydrogels may not only improve cell adhesion but also influence stem cell differentiation. In this study, PSBMA hydrogels were modified with RGD or RGD/QK peptides and characterized. Multipotent human adipose-tissue derived stem cells (hASCs) were seeded to peptides-grafted PSBMA hydrogels, and the cell adhesion, proliferentiation, and differentiation of hASCs on hydrogels were demonstrated. The results showed that grafting of RGD or RGD/QK peptides improved hASCs adhesion, while the mechanical strength of peptides-grafted PSBMA hydrogels was decreased. Incorporation of RGD or RGD/QK peptides did not influence the adsorption adsorption behaviors of PSBMA hydrogels. Relative to RGD-grafted PSBMA hydrogels, the RGD/QK incorporation further improved hASCs differentiated into adipogenic lineage, while RGD or RGD/QK additions had no substantial effects on osteogenesis under osteogenic induction. Moreover, the endothelial differentiation was also enhanced when hASCs cultured on RGD/QK-modified PSBMA hydrogels. In conclusion, the graftings of RGD and RGD/QK did not interfere the nonfouling property of PSBMA hydrogels. The incorporation of RGD/QK peptides can improve hASCs differentiate toward adipogenic/endothelial lineages.

AB - The superhydrophilic and ultralow biofouling properties as well as the resistance to foreign-body reaction make zwitterionic polymer promising in biomedical applications. Incorporation of arginine-glycine-aspartate (RGD) sequence and/or vascular endothelial growth factor mimicking (QK) peptide to zwitterionic poly(sulfobetaine methacrylate) (PSBMA) hydrogels may not only improve cell adhesion but also influence stem cell differentiation. In this study, PSBMA hydrogels were modified with RGD or RGD/QK peptides and characterized. Multipotent human adipose-tissue derived stem cells (hASCs) were seeded to peptides-grafted PSBMA hydrogels, and the cell adhesion, proliferentiation, and differentiation of hASCs on hydrogels were demonstrated. The results showed that grafting of RGD or RGD/QK peptides improved hASCs adhesion, while the mechanical strength of peptides-grafted PSBMA hydrogels was decreased. Incorporation of RGD or RGD/QK peptides did not influence the adsorption adsorption behaviors of PSBMA hydrogels. Relative to RGD-grafted PSBMA hydrogels, the RGD/QK incorporation further improved hASCs differentiated into adipogenic lineage, while RGD or RGD/QK additions had no substantial effects on osteogenesis under osteogenic induction. Moreover, the endothelial differentiation was also enhanced when hASCs cultured on RGD/QK-modified PSBMA hydrogels. In conclusion, the graftings of RGD and RGD/QK did not interfere the nonfouling property of PSBMA hydrogels. The incorporation of RGD/QK peptides can improve hASCs differentiate toward adipogenic/endothelial lineages.

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