Zinc oxide nanoparticles impair bacterial clearance by macrophages

Chia Der Lin, Yu Yi Kou, Chin Yu Liao, Ching Hao Li, Shiao Ping Huang, Yu Wen Cheng, Wei Chih Liao, Hao Xiang Chen, Pei Ling Wu, Jaw Jou Kang, Chen Chen Lee, Chih Ho Lai

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aim: The extensive development of nanoparticles (NPs) and their widespread employment in daily life have led to an increase in environmental concentrations of substances that may pose a biohazard to humans. The aim of this work was to examine the effects of zinc oxide nanoparticles (ZnO-NPs) on the host's pulmonary immune system response to nontypeable Haemophilus influenzae (NTHi) infection. Materials & Methods: A murine infection model was employed to assess pulmonary inflammation and bacterial clearance in response to exposure to ZnO-NPs. The molecular mechanisms underlying ZnO-NP-impaired macrophage activation were investigated. Results: Treatment with ZnO-NPs impaired macrophage activation, leading to a delay in NTHi clearance in the bronchial alveolar lavage fluids and lungs. Exposure to ZnO-NPs followed by NTHi challenge decreased levels of nitric oxide compared with NTHi infection alone. The effects of ZnO-NPs involved downregulation of NTHi-activated expression of inducible nitric oxide synthase and the translocation of active NF-kB into the nucleus. Conclusion: These results demonstrate that exposure to ZnO-NPs can impair innate immune responses and attenuate macrophage responses to bacterial infection.

Original languageEnglish
Pages (from-to)1327-1339
Number of pages13
JournalNanomedicine
Volume9
Issue number9
DOIs
Publication statusPublished - Jul 1 2014

Fingerprint

Zinc Oxide
Macrophages
Zinc oxide
Nanoparticles
contagious disease
influenza
zinc
oxide
Haemophilus influenzae
Haemophilus Infections
activation
Macrophage Activation
Nitric oxide
nitric oxide
Biohazards
Chemical activation
Hazardous Substances
Lung
nanoparticle
NF-kappa B

Keywords

  • bronchial alveolar lavage fluid
  • macrophage
  • nitric oxide
  • nontypeable Haemophilus influenzae
  • zinc oxide nanoparticles

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development
  • Medicine(all)

Cite this

Lin, C. D., Kou, Y. Y., Liao, C. Y., Li, C. H., Huang, S. P., Cheng, Y. W., ... Lai, C. H. (2014). Zinc oxide nanoparticles impair bacterial clearance by macrophages. Nanomedicine, 9(9), 1327-1339. https://doi.org/10.2217/nnm.14.48

Zinc oxide nanoparticles impair bacterial clearance by macrophages. / Lin, Chia Der; Kou, Yu Yi; Liao, Chin Yu; Li, Ching Hao; Huang, Shiao Ping; Cheng, Yu Wen; Liao, Wei Chih; Chen, Hao Xiang; Wu, Pei Ling; Kang, Jaw Jou; Lee, Chen Chen; Lai, Chih Ho.

In: Nanomedicine, Vol. 9, No. 9, 01.07.2014, p. 1327-1339.

Research output: Contribution to journalArticle

Lin, CD, Kou, YY, Liao, CY, Li, CH, Huang, SP, Cheng, YW, Liao, WC, Chen, HX, Wu, PL, Kang, JJ, Lee, CC & Lai, CH 2014, 'Zinc oxide nanoparticles impair bacterial clearance by macrophages', Nanomedicine, vol. 9, no. 9, pp. 1327-1339. https://doi.org/10.2217/nnm.14.48
Lin, Chia Der ; Kou, Yu Yi ; Liao, Chin Yu ; Li, Ching Hao ; Huang, Shiao Ping ; Cheng, Yu Wen ; Liao, Wei Chih ; Chen, Hao Xiang ; Wu, Pei Ling ; Kang, Jaw Jou ; Lee, Chen Chen ; Lai, Chih Ho. / Zinc oxide nanoparticles impair bacterial clearance by macrophages. In: Nanomedicine. 2014 ; Vol. 9, No. 9. pp. 1327-1339.
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