Zinc ion acts as a cofactor for serine/threonine kinase MST3 and has a distinct role in autophosphorylation of MST3

Te Jung Lu, Chi Ying F Huang, Chiun Jye Yuan, Yuan Chii Lee, Tzeng Horng Leu, Wen Chang Chang, Te Ling Lu, Wen Yih Jeng, Ming Derg Lai

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We examined the metal ion cofactor preference for MST3 (mammalian Ste20-like kinase 3) of the Ste20 serine/threonine kinase family. Four metal ions (Mg+2, Mn+2, Zn2+, and Co2+) activate endogenous, exogenous, and baculovirus-expressed recombinant MST3 within the physiological concentration range. In contrast, Fe+2 and Ca+2 do not function as MST3 cofactors. Mn2+, Co 2+, and Mg2+-dependent autophosphorylation of MST3 is mainly on threonine residue while Zn2+-stimulated MST3 autophosphorylation is on both serine and threonine residues. The distinct autophosphorylation pattern on MST3 suggests that MST3 may exert various types of kinase reactions depending on the type of metal ion cofactor used. To our knowledge, this is the first report showing Zn2+ as the metal ion cofactor of a recombinant serine/threonine kinase.

Original languageEnglish
Pages (from-to)1306-1313
Number of pages8
JournalJournal of Inorganic Biochemistry
Volume99
Issue number6
DOIs
Publication statusPublished - Jun 2005

Fingerprint

Protein-Serine-Threonine Kinases
Zinc
Phosphotransferases
Ions
Metal ions
Metals
Threonine
Baculoviridae
Serine

Keywords

  • Autophosphorylation
  • Cobalt
  • Cofactor
  • Kinase
  • MST3
  • Zinc

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Zinc ion acts as a cofactor for serine/threonine kinase MST3 and has a distinct role in autophosphorylation of MST3. / Lu, Te Jung; Huang, Chi Ying F; Yuan, Chiun Jye; Lee, Yuan Chii; Leu, Tzeng Horng; Chang, Wen Chang; Lu, Te Ling; Jeng, Wen Yih; Lai, Ming Derg.

In: Journal of Inorganic Biochemistry, Vol. 99, No. 6, 06.2005, p. 1306-1313.

Research output: Contribution to journalArticle

Lu, Te Jung ; Huang, Chi Ying F ; Yuan, Chiun Jye ; Lee, Yuan Chii ; Leu, Tzeng Horng ; Chang, Wen Chang ; Lu, Te Ling ; Jeng, Wen Yih ; Lai, Ming Derg. / Zinc ion acts as a cofactor for serine/threonine kinase MST3 and has a distinct role in autophosphorylation of MST3. In: Journal of Inorganic Biochemistry. 2005 ; Vol. 99, No. 6. pp. 1306-1313.
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AU - Leu, Tzeng Horng

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