Zinc finger protein 219-like (ZNF219L) and Sox9a regulate synuclein-γ2 (sncgb) expression in the developing notochord of zebrafish

Huang Wei Lien, Chung Hsiang Yang, Chia Hsiung Cheng, Yung Feng Liao, Yu San Han, Chang Jen Huang

Research output: Contribution to journalArticle

Abstract

Zebrafish synuclein-γ2 (sncgb) has been reported to be expressed specifically in the notochord. However, the mechanism by which the sncgb gene promoter is regulated has not been described. In this paper, we demonstrate that Zinc finger protein 219-like (ZNF219L) and sox9a are involved in the regulation of sncgb gene expression. Furthermore, we observed that over-expression of both ZNF219L and Sox9a resulted in increased sncgb expression. In addition, ZNF219L is physically associated with Sox9a, and simultaneous morpholino knockdown of znf219L and sox9a caused a synergistic decrease of sncgb expression in the notochord. Taken together, our results reveal that coordination of ZNF219L with Sox9a is involved in the regulation of notochord-specific expression of sncgb.

Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume442
Issue number3-4
DOIs
Publication statusPublished - Dec 13 2013

Fingerprint

Synucleins
Notochord
Zinc Fingers
Zebrafish
Zinc
Proteins
Morpholinos
Gene Expression Regulation
Gene expression
Genes

Keywords

  • Notochord
  • Sox9a
  • Synuclein-γ2 (sncgb)
  • Zebrafish
  • ZNF219-like (ZNF219L)

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Zinc finger protein 219-like (ZNF219L) and Sox9a regulate synuclein-γ2 (sncgb) expression in the developing notochord of zebrafish. / Lien, Huang Wei; Yang, Chung Hsiang; Cheng, Chia Hsiung; Liao, Yung Feng; Han, Yu San; Huang, Chang Jen.

In: Biochemical and Biophysical Research Communications, Vol. 442, No. 3-4, 13.12.2013, p. 189-194.

Research output: Contribution to journalArticle

Lien, Huang Wei ; Yang, Chung Hsiang ; Cheng, Chia Hsiung ; Liao, Yung Feng ; Han, Yu San ; Huang, Chang Jen. / Zinc finger protein 219-like (ZNF219L) and Sox9a regulate synuclein-γ2 (sncgb) expression in the developing notochord of zebrafish. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 442, No. 3-4. pp. 189-194.
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AU - Huang, Chang Jen

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