Zebrafish cyclin Dx is required for development of motor neuron progenitors, and its expression is regulated by hypoxia-inducible factor 2α

Huang Wei Lien, Rey Yue Yuan, Chih Ming Chou, Yi Chung Chen, Chin Chun Hung, Chin Hwa Hu, Sheng Ping L Hwang, Pung Pung Hwang, Chia Ning Shen, Chih Lung Chen, Chia Hsiung Cheng, Chang Jen Huang

Research output: Contribution to journalArticle

Abstract

Cyclins play a central role in cell-cycle regulation; in mammals, the D family of cyclins consists of cyclin D1, D2, and D3. In Xenopus, only homologs of cyclins D1 and D2 have been reported, while a novel cyclin, cyclin Dx (ccndx), was found to be required for the maintenance of motor neuron progenitors during embryogenesis. It remains unknown whether zebrafish possess cyclin D3 or cyclin Dx. In this study, we identified a zebrafish ccndx gene encoding a protein which can form a complex with Cdk4. Through whole-mount in situ hybridization, we observed that zccndx mRNA is expressed in the motor neurons of hindbrain and spinal cord during development. Analysis of a 4-kb promoter sequence of the zccndx gene revealed the presence of HRE sites, which can be regulated by HIF2α. Morpholino knockdown of zebrafish Hif2α and cyclin Dx resulted in the abolishment of isl1 and oligo2 expression in the precursors of motor neurons, and also disrupted axon growth. Overexpression of cyclin Dx mRNA in Hif2α morphants partially rescued zccndx expression. Taken together, our data indicate that zebrafish cyclin Dx plays a role in maintaining the precursors of motor neurons.

Original languageEnglish
Article number28297
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Jun 21 2016

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Cyclins
Motor Neurons
Zebrafish
Cyclin D3
Cyclin D2
Cyclin D1
Cyclin D
endothelial PAS domain-containing protein 1
Morpholinos
Rhombencephalon
Messenger RNA
Xenopus
Genes
Embryonic Development
In Situ Hybridization
Axons
Mammals
Spinal Cord
Cell Cycle
Maintenance

ASJC Scopus subject areas

  • General

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Zebrafish cyclin Dx is required for development of motor neuron progenitors, and its expression is regulated by hypoxia-inducible factor 2α. / Lien, Huang Wei; Yuan, Rey Yue; Chou, Chih Ming; Chen, Yi Chung; Hung, Chin Chun; Hu, Chin Hwa; Hwang, Sheng Ping L; Hwang, Pung Pung; Shen, Chia Ning; Chen, Chih Lung; Cheng, Chia Hsiung; Huang, Chang Jen.

In: Scientific Reports, Vol. 6, 28297, 21.06.2016.

Research output: Contribution to journalArticle

Lien, Huang Wei ; Yuan, Rey Yue ; Chou, Chih Ming ; Chen, Yi Chung ; Hung, Chin Chun ; Hu, Chin Hwa ; Hwang, Sheng Ping L ; Hwang, Pung Pung ; Shen, Chia Ning ; Chen, Chih Lung ; Cheng, Chia Hsiung ; Huang, Chang Jen. / Zebrafish cyclin Dx is required for development of motor neuron progenitors, and its expression is regulated by hypoxia-inducible factor 2α. In: Scientific Reports. 2016 ; Vol. 6.
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