Z-DNA forms without an alternating purine-pyrimidine sequence in solution

Juli Feigon, Andrew H.J. Wang, Gijs A. Van Der Marel, Jacques H. Van Boom, Alexander Rich

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

Nuclear magnetic resonance spectra (proton and phosphorus-31) and ultraviolet absorption spectra of the DNA decamer d(br5CGbr 5CGATbr5CGbr5CG), in which the central two adenine-thymine base pairs are out of order with the rest of the purine-pyrimidine alternation sequence, indicate that under appropriate solvent conditions (high salt and methanol) the molecule undergoes a structural transition from a right-handed B-DNA conformation to a left-handed Z-DNA conformation. Measurements of the two-dimensional nuclear Overhauser effect on the decamer indicate that all of the guanines as well as the two equivalent thymines adopt the syn conformation.

Original languageEnglish
Pages (from-to)82-84
Number of pages3
JournalScience
Volume230
Issue number4721
DOIs
Publication statusPublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • General

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