Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis

An Ming Wang, Tzu Ting Huang, Kai Wen Hsu, Kuo Hung Huang, Wen Liang Fang, Muh Hwa Yang, Su Shun Lo, Chin Wen Chi, Jing Jer Lin, Tian-Shun Tsai

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Gastric cancer is the second leading cause of cancer-related death worldwide. Herein, we investigated the role of transcription factor Yin Yang 1 (YY1), a multi-functional protein, in tumorigenesis of gastric cancer cells. Results showed that YY1 contributed to gastric carcinogenesis of SC-M1 cells including growth, viability, and abilities of colony formation, migration, invasion, and tumorsphere formation. Levels of pluripotency genes CD44, Oct4, SOX-2, and Nanog were also up-regulated by YY1 in SC-M1 cells. Additionally, the 3'-untranslated region (3'-UTR) of YY1 mRNA was the target of microRNA-34 (miR-34) family consisting of miR-34a, miR-34b, and miR-34c. Overexpression of miR-34 family suppressed carcinogenesis through down-regulation of YY1 in NUGC-3 gastric cancer cells scarcely expressing miR-34 family. Alternatively, knockdown of miR-34 family promoted tumorigenesis via up-regulation of YY1 in SC-M1 and AZ521 gastric cancer cells with higher levels of miR-34 family. The miR-34 family also affected tumorsphere ultra-structure and inhibited the xenografted tumor growth as well as lung metastasis of SC-M1 cells through YY1. Expressions of miR-34a and miR-34c in gastric cancer tissues of patients were lower than those in normal tissues. Taken together, these results suggest that miR-34 family-YY1 axis plays an important role in the control of gastric carcinogenesis.

Original languageEnglish
Pages (from-to)5002-5016
Number of pages15
JournalOncotarget
Volume5
Issue number13
Publication statusPublished - 2014

Fingerprint

Yin-Yang
MicroRNAs
Stomach
Carcinogenesis
Stomach Neoplasms
Aptitude
3' Untranslated Regions
Growth
Neoplasms
Transcription Factors
Up-Regulation
Down-Regulation
Neoplasm Metastasis
Lung
Messenger RNA

Keywords

  • Carcinogenesis
  • Gastric cancer
  • miR-34 family
  • Pluripotency
  • YY1

ASJC Scopus subject areas

  • Oncology
  • Medicine(all)

Cite this

Wang, A. M., Huang, T. T., Hsu, K. W., Huang, K. H., Fang, W. L., Yang, M. H., ... Tsai, T-S. (2014). Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis. Oncotarget, 5(13), 5002-5016.

Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis. / Wang, An Ming; Huang, Tzu Ting; Hsu, Kai Wen; Huang, Kuo Hung; Fang, Wen Liang; Yang, Muh Hwa; Lo, Su Shun; Chi, Chin Wen; Lin, Jing Jer; Tsai, Tian-Shun.

In: Oncotarget, Vol. 5, No. 13, 2014, p. 5002-5016.

Research output: Contribution to journalArticle

Wang, AM, Huang, TT, Hsu, KW, Huang, KH, Fang, WL, Yang, MH, Lo, SS, Chi, CW, Lin, JJ & Tsai, T-S 2014, 'Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis', Oncotarget, vol. 5, no. 13, pp. 5002-5016.
Wang AM, Huang TT, Hsu KW, Huang KH, Fang WL, Yang MH et al. Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis. Oncotarget. 2014;5(13):5002-5016.
Wang, An Ming ; Huang, Tzu Ting ; Hsu, Kai Wen ; Huang, Kuo Hung ; Fang, Wen Liang ; Yang, Muh Hwa ; Lo, Su Shun ; Chi, Chin Wen ; Lin, Jing Jer ; Tsai, Tian-Shun. / Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis. In: Oncotarget. 2014 ; Vol. 5, No. 13. pp. 5002-5016.
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