YC-1, a potent antithrombotic agent, induces lipolysis through the PKA pathway in rat visceral fat cells

Chih Hui Chin, Feng-Chou Tsai, Sy Ping Chen, Ke Chuan Wang, Chao Chien Chang, Man Hui Pai, Tsorng Harn Fong

Research output: Contribution to journalArticle

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Abstract

This study investigated the effects of 3-(5′-hydroxymethyl-2′- furyl)-1-benzylindazole (YC-1), a soluble guanylyl cyclase (sGC) activator and potential antithrombotic agent, on lipolysis in isolated visceral fat cells of the rat. Visceral fat cells were isolated from epididymal fat pads of rats and treated with YC-1 at different doses and times. Glycerol release, and intracellular cAMP and cGMP levels were analyzed by specific kits. Moreover, several inhibitors or drugs were used to examine the signal transduction pathways of YC-1-induced lipolysis in adipocytes. Herein we report that YC-1 stimulated glycerol release in dose- and time-dependent manners. Intracellular cAMP and cGMP levels of adipocytes both increased in time-dependent manners, but elevation of the cGMP level was faster and higher than that of the cAMP level after YC-1 treatment. An sGC inhibitor (ODQ) inhibited YC-1-induced glycerol release, indicating the involvement of sGC in YC-1-induced lipolysis. Administration of insulin, an activator of type-3B phosphodiesterase (PDE-3B), attenuated YC-1-induced lipolysis, indicating that elevation of the cAMP level is an important step in the lipolytic effect of YC-1. In addition, YC-1-induced lipolysis was inhibited by a protein kinase A (PKA) inhibitor (KT5720) but not by a PKG inhibitor (KT5823), indicating that YC-1-induced lipolysis occurs through a PKA-dependent pathway. A Western blot analysis showed that extracellular signal-regulated kinase was not phosphorylated by YC-1 treatment. In conclusion, our results suggest that YC-1 might stimulate lipolysis via activation of sGC/cGMP and then activation of the cAMP/PKA signaling cascade in isolated rat visceral adipocytes.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalEuropean Journal of Pharmacology
Volume689
Issue number1-3
DOIs
Publication statusPublished - Aug 15 2012

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Fibrinolytic Agents
Intra-Abdominal Fat
Lipolysis
Cyclic AMP-Dependent Protein Kinases
Adipocytes
Glycerol
Type 3 Cyclic Nucleotide Phosphodiesterases
Extracellular Signal-Regulated MAP Kinases
Protein Kinase Inhibitors
Adipose Tissue
Signal Transduction
Western Blotting
Insulin
Soluble Guanylyl Cyclase
Pharmaceutical Preparations

Keywords

  • Adipocytes
  • Lipolysis
  • PKA (Protein kinase A)
  • YC-1

ASJC Scopus subject areas

  • Pharmacology

Cite this

YC-1, a potent antithrombotic agent, induces lipolysis through the PKA pathway in rat visceral fat cells. / Chin, Chih Hui; Tsai, Feng-Chou; Chen, Sy Ping; Wang, Ke Chuan; Chang, Chao Chien; Pai, Man Hui; Fong, Tsorng Harn.

In: European Journal of Pharmacology, Vol. 689, No. 1-3, 15.08.2012, p. 1-7.

Research output: Contribution to journalArticle

Chin, Chih Hui ; Tsai, Feng-Chou ; Chen, Sy Ping ; Wang, Ke Chuan ; Chang, Chao Chien ; Pai, Man Hui ; Fong, Tsorng Harn. / YC-1, a potent antithrombotic agent, induces lipolysis through the PKA pathway in rat visceral fat cells. In: European Journal of Pharmacology. 2012 ; Vol. 689, No. 1-3. pp. 1-7.
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AU - Wang, Ke Chuan

AU - Chang, Chao Chien

AU - Pai, Man Hui

AU - Fong, Tsorng Harn

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