Yam (Dioscorea batatas) tuber mucilage exhibited antioxidant activities in vitro

Wen Chi Hou, Feng-Lin Hsu, Mei Hsien Lee

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

The yam (Dioscorea batatas Decne) tuber mucilage (YTM) was extracted and partially purified by SDS and heating treatments. This purified YTM exhibited antioxidant activities in a series of in vitro tests, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical (half-inhibition concentration, IC50, was 0.86 mg/mL) and hydroxyl radical (IC50 was 22 μg/mL) scavenging activity assays, reducing power test, anti-lipid peroxidation and anti-human low density lipoprotein peroxidation tests (IC50 was 145.46/μg/mL) using butylated hydroxytoluene (BHT), reduced glutathione, or ascorbic acid for comparisons. With electron paramagnetic resonance (EPR) spectrometry for DPPH radical detection, the intensities of the EPR signals were decreased by the increased amounts of YTM added (IC50 was 1.62 mg/mL). These results suggest that mucilage of yam tuber might play roles as antiradicals and antioxidants.

Original languageEnglish
Pages (from-to)1072-1076
Number of pages5
JournalPlanta Medica
Volume68
Issue number12
DOIs
Publication statusPublished - Dec 1 2002

Fingerprint

Dioscorea polystachya
Dioscorea
mucilages
yams
Inhibitory Concentration 50
Paramagnetic resonance
inhibitory concentration 50
tubers
Antioxidants
antioxidant activity
Butylated Hydroxytoluene
Scavenging
electron paramagnetic resonance spectroscopy
Electron Spin Resonance Spectroscopy
LDL Lipoproteins
Hydroxyl Radical
Spectrometry
Ascorbic Acid
Glutathione
Assays

Keywords

  • 1,1-diphenyl-2-picrylhydrazyl (DPPH)
  • Antioxidant
  • Dioscorea batatas
  • Dioscoreaceae
  • Electron paramagnetic resonance (EPR)
  • Hydroxyl radical
  • Mucilage

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Yam (Dioscorea batatas) tuber mucilage exhibited antioxidant activities in vitro. / Hou, Wen Chi; Hsu, Feng-Lin; Lee, Mei Hsien.

In: Planta Medica, Vol. 68, No. 12, 01.12.2002, p. 1072-1076.

Research output: Contribution to journalArticle

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