Wound healing effects of porcine placental extracts on rats with thermal injury

Chieh H. Wu, G. Y. Chang, W. C. Chang, C. T. Hsu, R. S. Chen

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Placental extracts have been used as Chinese folk medicines to accelerate wound healing. However, the molecular mechanism of placental extracts on wound healing has not been identifed. It is known that fibroblast growth factors (FGF) and transforming growth factors (TGF) are two key factors involved in wound healing. Objectives: To determine the molecular mechanism of placental extracts on wound healing. Methods: The protein levels of both growth factors in rat skins with thermal injury were therefore studied to explore the molecular mechanism of placental extracts on wound healing. As cell proliferation is essential for wound healing, effects of placental extracts on fibroblast proliferation were also determined. Results: As compared with the controls, the S phase of fibroblasts was significantly increased by 1·5-, 1·7- and 4·7-fold for 1, 10 and 30 mg mL-1 of placental extracts, respectively. The increase of the S phase was not due to the minute amount of sex hormones in the placental extracts as the addition of equivalent amounts of hormones showed no increase of the S phase. In addition, a 2·5-fold increase of TGF-β1 in wound skin biopsy was noticed with 30 mg mL-1 of porcine placental extracts. The FGF levels in the wound skin receiving 30 mg mL-1 of porcine placental extracts were also significantly increased compared with the controls. Conclusions: These ex vivo data support the observation that the application of 30 mg mL-1 of placental extracts reduced the wound healing time by about 50%. To the best of our knowledge, this is the first report to explore the molecular mechanisms of porcine placental extracts on wound healing. These results may provide the insight into the potential use of porcine placental extracts as an alternative medicine for accelerating wound healing.

Original languageEnglish
Pages (from-to)236-245
Number of pages10
JournalBritish Journal of Dermatology
Volume148
Issue number2
DOIs
Publication statusPublished - Feb 1 2003
Externally publishedYes

Fingerprint

Placental Extracts
Wound Healing
Swine
Hot Temperature
Wounds and Injuries
S Phase
Fibroblast Growth Factors
Transforming Growth Factors
Skin
Fibroblasts
Gonadal Steroid Hormones
Traditional Medicine
Complementary Therapies

Keywords

  • Fibroblast growth factor
  • Placental extracts
  • Transforming growth factor-β1
  • Wound healing

ASJC Scopus subject areas

  • Dermatology

Cite this

Wound healing effects of porcine placental extracts on rats with thermal injury. / Wu, Chieh H.; Chang, G. Y.; Chang, W. C.; Hsu, C. T.; Chen, R. S.

In: British Journal of Dermatology, Vol. 148, No. 2, 01.02.2003, p. 236-245.

Research output: Contribution to journalArticle

Wu, Chieh H. ; Chang, G. Y. ; Chang, W. C. ; Hsu, C. T. ; Chen, R. S. / Wound healing effects of porcine placental extracts on rats with thermal injury. In: British Journal of Dermatology. 2003 ; Vol. 148, No. 2. pp. 236-245.
@article{e4939dc42e444c529f82bdf05202724d,
title = "Wound healing effects of porcine placental extracts on rats with thermal injury",
abstract = "Background: Placental extracts have been used as Chinese folk medicines to accelerate wound healing. However, the molecular mechanism of placental extracts on wound healing has not been identifed. It is known that fibroblast growth factors (FGF) and transforming growth factors (TGF) are two key factors involved in wound healing. Objectives: To determine the molecular mechanism of placental extracts on wound healing. Methods: The protein levels of both growth factors in rat skins with thermal injury were therefore studied to explore the molecular mechanism of placental extracts on wound healing. As cell proliferation is essential for wound healing, effects of placental extracts on fibroblast proliferation were also determined. Results: As compared with the controls, the S phase of fibroblasts was significantly increased by 1·5-, 1·7- and 4·7-fold for 1, 10 and 30 mg mL-1 of placental extracts, respectively. The increase of the S phase was not due to the minute amount of sex hormones in the placental extracts as the addition of equivalent amounts of hormones showed no increase of the S phase. In addition, a 2·5-fold increase of TGF-β1 in wound skin biopsy was noticed with 30 mg mL-1 of porcine placental extracts. The FGF levels in the wound skin receiving 30 mg mL-1 of porcine placental extracts were also significantly increased compared with the controls. Conclusions: These ex vivo data support the observation that the application of 30 mg mL-1 of placental extracts reduced the wound healing time by about 50{\%}. To the best of our knowledge, this is the first report to explore the molecular mechanisms of porcine placental extracts on wound healing. These results may provide the insight into the potential use of porcine placental extracts as an alternative medicine for accelerating wound healing.",
keywords = "Fibroblast growth factor, Placental extracts, Transforming growth factor-β1, Wound healing",
author = "Wu, {Chieh H.} and Chang, {G. Y.} and Chang, {W. C.} and Hsu, {C. T.} and Chen, {R. S.}",
year = "2003",
month = "2",
day = "1",
doi = "10.1046/j.1365-2133.2003.05164.x",
language = "English",
volume = "148",
pages = "236--245",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Wound healing effects of porcine placental extracts on rats with thermal injury

AU - Wu, Chieh H.

AU - Chang, G. Y.

AU - Chang, W. C.

AU - Hsu, C. T.

AU - Chen, R. S.

PY - 2003/2/1

Y1 - 2003/2/1

N2 - Background: Placental extracts have been used as Chinese folk medicines to accelerate wound healing. However, the molecular mechanism of placental extracts on wound healing has not been identifed. It is known that fibroblast growth factors (FGF) and transforming growth factors (TGF) are two key factors involved in wound healing. Objectives: To determine the molecular mechanism of placental extracts on wound healing. Methods: The protein levels of both growth factors in rat skins with thermal injury were therefore studied to explore the molecular mechanism of placental extracts on wound healing. As cell proliferation is essential for wound healing, effects of placental extracts on fibroblast proliferation were also determined. Results: As compared with the controls, the S phase of fibroblasts was significantly increased by 1·5-, 1·7- and 4·7-fold for 1, 10 and 30 mg mL-1 of placental extracts, respectively. The increase of the S phase was not due to the minute amount of sex hormones in the placental extracts as the addition of equivalent amounts of hormones showed no increase of the S phase. In addition, a 2·5-fold increase of TGF-β1 in wound skin biopsy was noticed with 30 mg mL-1 of porcine placental extracts. The FGF levels in the wound skin receiving 30 mg mL-1 of porcine placental extracts were also significantly increased compared with the controls. Conclusions: These ex vivo data support the observation that the application of 30 mg mL-1 of placental extracts reduced the wound healing time by about 50%. To the best of our knowledge, this is the first report to explore the molecular mechanisms of porcine placental extracts on wound healing. These results may provide the insight into the potential use of porcine placental extracts as an alternative medicine for accelerating wound healing.

AB - Background: Placental extracts have been used as Chinese folk medicines to accelerate wound healing. However, the molecular mechanism of placental extracts on wound healing has not been identifed. It is known that fibroblast growth factors (FGF) and transforming growth factors (TGF) are two key factors involved in wound healing. Objectives: To determine the molecular mechanism of placental extracts on wound healing. Methods: The protein levels of both growth factors in rat skins with thermal injury were therefore studied to explore the molecular mechanism of placental extracts on wound healing. As cell proliferation is essential for wound healing, effects of placental extracts on fibroblast proliferation were also determined. Results: As compared with the controls, the S phase of fibroblasts was significantly increased by 1·5-, 1·7- and 4·7-fold for 1, 10 and 30 mg mL-1 of placental extracts, respectively. The increase of the S phase was not due to the minute amount of sex hormones in the placental extracts as the addition of equivalent amounts of hormones showed no increase of the S phase. In addition, a 2·5-fold increase of TGF-β1 in wound skin biopsy was noticed with 30 mg mL-1 of porcine placental extracts. The FGF levels in the wound skin receiving 30 mg mL-1 of porcine placental extracts were also significantly increased compared with the controls. Conclusions: These ex vivo data support the observation that the application of 30 mg mL-1 of placental extracts reduced the wound healing time by about 50%. To the best of our knowledge, this is the first report to explore the molecular mechanisms of porcine placental extracts on wound healing. These results may provide the insight into the potential use of porcine placental extracts as an alternative medicine for accelerating wound healing.

KW - Fibroblast growth factor

KW - Placental extracts

KW - Transforming growth factor-β1

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=0037328877&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037328877&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2133.2003.05164.x

DO - 10.1046/j.1365-2133.2003.05164.x

M3 - Article

C2 - 12588374

AN - SCOPUS:0037328877

VL - 148

SP - 236

EP - 245

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 2

ER -