Wound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium

Ray Neng Chen, Lin Wen Lee, Ling Chun Chen, Hsiu O. Ho, Shiao Chuan Lui, Ming Thau Sheu, Ching-Hua Su

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The extraction residue of the Ganoderma fruiting body, named sacchachitin, has been demonstrated to have the potential to enhance cutaneous wound healing by inducing cell proliferation. In this study, a nanogel formed from micronized sacchachitin (mSC) was investigated for the potential treatment of superficial chemical corneal burns. Reportedly, mSC has been produced successfully and its chemical properties confirmed, and physical and rheological properties characterized. An in vitro cell proliferation study has revealed that at the concentrations of 200, 300, and 400 μg/mL, mSC nanogel significantly increased Statens Seruminstitut rabbit corneal (SIRC) cell proliferation after 24 hours of incubation. In cell migration assay, migration of SIRC cell to wound closure was observed after 24 hours of incubation with the addition of 200 μg/mL mSC of nanogel. In an animal study, acceleration of corneal wound healing was probably due to the inhibition of proteolysis. In conclusion, the findings of this study substantiate the potential application of sacchachitin in the form of mSC nanogel for the treatment of superficial corneal injuries.

Original languageEnglish
Pages (from-to)4697-4706
Number of pages10
JournalInternational Journal of Nanomedicine
Volume7
DOIs
Publication statusPublished - 2012

Keywords

  • Corneal wound healing
  • Matrix metalloproteinase
  • Sacchachitin

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

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