Wogonin inhibits IL-6-induced angiogenesis via down-regulation of VEGF and VEGFR-1, not VEGFR-2

Chiu Mei Lin, Hang Chang, Yeh Hsu Chen, I. Hsing Wu, Jen Hwey Chiu

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

So far, no antiangiogenic activity of wogonin, a flavonoid, on human umbilical vein endothelial cells (HUVECs) has been demonstrated. The aim of this study was to investigate the effects of wogonin on IL-6-induced angiogenesis in HUVEC cultures and chorioallantoic membrane (CAM) neovascularization. The in vivo CAM model was applied to evaluate the percentage of new vessel formations, followed by measurement of endothelial migration and tube formation in HUVEC cultures. The results revealed that wogonin (10-8-10-5M) concentration-dependently inhibited IL-6-induced angiogenesis. The signaling pathway was through down-regulation of the autocrine loop of VEGF and VEGFR-1, but not of VEGFR-2. Furthermore, the regulating loop of the IL-6 receptor complex was also attenuated via expression of sIL-6Rα and gp130, but not of IL-6/IL-6R binding density. We conclude that wogonin is a suppressive agent of the autoregulated loop of VEGF, VEGFR-1 and the IL-6 receptor complex.

Original languageEnglish
Pages (from-to)1305-1310
Number of pages6
JournalPlanta Medica
Volume72
Issue number14
DOIs
Publication statusPublished - Nov 2006

Keywords

  • Angiogenesis
  • IL-6
  • IL-6R
  • VEGF
  • VEGFR-1
  • Wogonin

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

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