Wild bitter melon (Momordica charantia Linn. var. abbreviata Ser.) extract and its bioactive components suppress Propionibacterium acnes-induced inflammation

Chin Hsu, Tsung Hsien Tsai, You Yi Li, Wen Huey Wu, Ching Jang Huang, Po Jung Tsai

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

In this study, we aimed to evaluate the inhibitory effect of wild bitter melons (WBM; Momordica charantia Linn. var. abbreviata Ser.) on Propionibacterium acnes-induced inflammation and to identify the bioactive components. Our results showed that ethyl acetate (EA) extract of WBM fruit in vitro potently suppressed pro-inflammatory cytokine and matrix metalloproteinase (MMP)-9 levels in P. acnes-stimulated THP-1 cells. Furthermore, concomitant intradermal injection of WBM EA extract in mice effectively attenuated P. acnes-induced ear swelling and granulomatous inflammation. To further investigate the bioactive components, we found that both saponifiable (S) and nonsaponifiable (NS) fractions of WBM EA extract significantly suppressed pro-inflammatory cytokine and MMP-9 levels. Phytol and lutein, identified in the NS fraction, also inhibited cytokine production. Moreover, S and NS fractions of EA extract, phytol and lutein, activated peroxisome proliferator-activated receptor (PPAR) α and β in the transactivation assay. Our results suggested that PPARα or PPARγ signalling may contribute, at least in part, to the anti-inflammatory activity of WBM.

Original languageEnglish
Pages (from-to)976-984
Number of pages9
JournalFood Chemistry
Volume135
Issue number3
DOIs
Publication statusPublished - Dec 1 2012

Fingerprint

Momordica charantia
Propionibacterium acnes
Abbreviata
Peroxisome Proliferator-Activated Receptors
inflammation
Phytol
Inflammation
Lutein
gelatinase B
cytokines
Matrix Metalloproteinase 9
extracts
Cytokines
lutein
Intradermal Injections
fruit extracts
transcriptional activation
Fruits
anti-inflammatory activity
Transcriptional Activation

Keywords

  • Anti-inflammation
  • Peroxisome proliferator-activated receptor
  • Propionibacterium acnes
  • Wild bitter melon

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Wild bitter melon (Momordica charantia Linn. var. abbreviata Ser.) extract and its bioactive components suppress Propionibacterium acnes-induced inflammation. / Hsu, Chin; Tsai, Tsung Hsien; Li, You Yi; Wu, Wen Huey; Huang, Ching Jang; Tsai, Po Jung.

In: Food Chemistry, Vol. 135, No. 3, 01.12.2012, p. 976-984.

Research output: Contribution to journalArticle

Hsu, Chin ; Tsai, Tsung Hsien ; Li, You Yi ; Wu, Wen Huey ; Huang, Ching Jang ; Tsai, Po Jung. / Wild bitter melon (Momordica charantia Linn. var. abbreviata Ser.) extract and its bioactive components suppress Propionibacterium acnes-induced inflammation. In: Food Chemistry. 2012 ; Vol. 135, No. 3. pp. 976-984.
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