Which test is a better strategy to determine the outcome of atypical glandular cell-categorized Pap smears? Immunocytochemical p16INK4A expression or human papillomavirus test - A retrospective cohort study

Su Feng Chen, Shih Fang Yang, Tang Yuan Chu, Hung Cheng Lai, Ya Wen Lin, Chien Yu Bai, Shin Nieh

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective. This study was to correlate high-risk human papillomavirus (HR-HPV) viral load to p16INK4A (p16) expression in atypical glandular cell (AGC)-categorized Pap smears with follow-up biopsies for elucidating their relationships. Methods. We enrolled 36 AGC-categorized Pap smears with subsequent follow-up biopsies. HR-HPV viral load was determined by Hybrid Capture II assay in each AGC-diagnosed Pap smear. Both smears and biopsies were immunostained with a primary anti-p16 antibody, clone E6H4. Correlations between HR-HPV viral load in each AGC-diagnosed Pap smear and p16 expression of smears with follow-up biopsies were performed. Results. Comparative analysis of two tests disclosed both consistencies and discrepancies. There were significant differences (P = 0.02) between negative or weak p16 expression of Pap smears with the presence of reactive lesion or LSILs/CIN1s in follow-up biopsies and negative HR-HPV viral load. However, no significant difference (P = 0.317) was found between p16 expression of Pap smears with the presence of HSIL/CIN2, 3 and AIS or adenocarcinoma in follow-up biopsies and high HR-HPV viral load. In addition, there were significant differences (P = 0.012) in specificity, but no significant differences were found in sensitivity (P = 0.604), positive and negative predictive value (P = 0.066 and 0.264) between p16 immunoexpression and HR-HPV viral load. Conclusions. Pathogenic activity of HR-HPV was indicated by p16 expression on smears and tissue sections, which appears to be a better strategy than HR-HPV viral load test for the detection of clinically insignificant lesions from AGC-categorized Pap smears.

Original languageEnglish
Pages (from-to)578-584
Number of pages7
JournalGynecologic Oncology
Volume99
Issue number3
DOIs
Publication statusPublished - Dec 2005
Externally publishedYes

Fingerprint

Papanicolaou Test
Viral Load
Cohort Studies
Retrospective Studies
Biopsy
Anti-Idiotypic Antibodies
Adenocarcinoma
Clone Cells

Keywords

  • Atypical glandular cells
  • Human papillomavirus
  • Immunocytochemistry
  • p16

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology

Cite this

Which test is a better strategy to determine the outcome of atypical glandular cell-categorized Pap smears? Immunocytochemical p16INK4A expression or human papillomavirus test - A retrospective cohort study. / Chen, Su Feng; Yang, Shih Fang; Chu, Tang Yuan; Lai, Hung Cheng; Lin, Ya Wen; Bai, Chien Yu; Nieh, Shin.

In: Gynecologic Oncology, Vol. 99, No. 3, 12.2005, p. 578-584.

Research output: Contribution to journalArticle

@article{25bf954c668342f5bb88c0184bfffc0d,
title = "Which test is a better strategy to determine the outcome of atypical glandular cell-categorized Pap smears? Immunocytochemical p16INK4A expression or human papillomavirus test - A retrospective cohort study",
abstract = "Objective. This study was to correlate high-risk human papillomavirus (HR-HPV) viral load to p16INK4A (p16) expression in atypical glandular cell (AGC)-categorized Pap smears with follow-up biopsies for elucidating their relationships. Methods. We enrolled 36 AGC-categorized Pap smears with subsequent follow-up biopsies. HR-HPV viral load was determined by Hybrid Capture II assay in each AGC-diagnosed Pap smear. Both smears and biopsies were immunostained with a primary anti-p16 antibody, clone E6H4. Correlations between HR-HPV viral load in each AGC-diagnosed Pap smear and p16 expression of smears with follow-up biopsies were performed. Results. Comparative analysis of two tests disclosed both consistencies and discrepancies. There were significant differences (P = 0.02) between negative or weak p16 expression of Pap smears with the presence of reactive lesion or LSILs/CIN1s in follow-up biopsies and negative HR-HPV viral load. However, no significant difference (P = 0.317) was found between p16 expression of Pap smears with the presence of HSIL/CIN2, 3 and AIS or adenocarcinoma in follow-up biopsies and high HR-HPV viral load. In addition, there were significant differences (P = 0.012) in specificity, but no significant differences were found in sensitivity (P = 0.604), positive and negative predictive value (P = 0.066 and 0.264) between p16 immunoexpression and HR-HPV viral load. Conclusions. Pathogenic activity of HR-HPV was indicated by p16 expression on smears and tissue sections, which appears to be a better strategy than HR-HPV viral load test for the detection of clinically insignificant lesions from AGC-categorized Pap smears.",
keywords = "Atypical glandular cells, Human papillomavirus, Immunocytochemistry, p16",
author = "Chen, {Su Feng} and Yang, {Shih Fang} and Chu, {Tang Yuan} and Lai, {Hung Cheng} and Lin, {Ya Wen} and Bai, {Chien Yu} and Shin Nieh",
year = "2005",
month = "12",
doi = "10.1016/j.ygyno.2005.06.060",
language = "English",
volume = "99",
pages = "578--584",
journal = "Gynecologic Oncology",
issn = "0090-8258",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Which test is a better strategy to determine the outcome of atypical glandular cell-categorized Pap smears? Immunocytochemical p16INK4A expression or human papillomavirus test - A retrospective cohort study

AU - Chen, Su Feng

AU - Yang, Shih Fang

AU - Chu, Tang Yuan

AU - Lai, Hung Cheng

AU - Lin, Ya Wen

AU - Bai, Chien Yu

AU - Nieh, Shin

PY - 2005/12

Y1 - 2005/12

N2 - Objective. This study was to correlate high-risk human papillomavirus (HR-HPV) viral load to p16INK4A (p16) expression in atypical glandular cell (AGC)-categorized Pap smears with follow-up biopsies for elucidating their relationships. Methods. We enrolled 36 AGC-categorized Pap smears with subsequent follow-up biopsies. HR-HPV viral load was determined by Hybrid Capture II assay in each AGC-diagnosed Pap smear. Both smears and biopsies were immunostained with a primary anti-p16 antibody, clone E6H4. Correlations between HR-HPV viral load in each AGC-diagnosed Pap smear and p16 expression of smears with follow-up biopsies were performed. Results. Comparative analysis of two tests disclosed both consistencies and discrepancies. There were significant differences (P = 0.02) between negative or weak p16 expression of Pap smears with the presence of reactive lesion or LSILs/CIN1s in follow-up biopsies and negative HR-HPV viral load. However, no significant difference (P = 0.317) was found between p16 expression of Pap smears with the presence of HSIL/CIN2, 3 and AIS or adenocarcinoma in follow-up biopsies and high HR-HPV viral load. In addition, there were significant differences (P = 0.012) in specificity, but no significant differences were found in sensitivity (P = 0.604), positive and negative predictive value (P = 0.066 and 0.264) between p16 immunoexpression and HR-HPV viral load. Conclusions. Pathogenic activity of HR-HPV was indicated by p16 expression on smears and tissue sections, which appears to be a better strategy than HR-HPV viral load test for the detection of clinically insignificant lesions from AGC-categorized Pap smears.

AB - Objective. This study was to correlate high-risk human papillomavirus (HR-HPV) viral load to p16INK4A (p16) expression in atypical glandular cell (AGC)-categorized Pap smears with follow-up biopsies for elucidating their relationships. Methods. We enrolled 36 AGC-categorized Pap smears with subsequent follow-up biopsies. HR-HPV viral load was determined by Hybrid Capture II assay in each AGC-diagnosed Pap smear. Both smears and biopsies were immunostained with a primary anti-p16 antibody, clone E6H4. Correlations between HR-HPV viral load in each AGC-diagnosed Pap smear and p16 expression of smears with follow-up biopsies were performed. Results. Comparative analysis of two tests disclosed both consistencies and discrepancies. There were significant differences (P = 0.02) between negative or weak p16 expression of Pap smears with the presence of reactive lesion or LSILs/CIN1s in follow-up biopsies and negative HR-HPV viral load. However, no significant difference (P = 0.317) was found between p16 expression of Pap smears with the presence of HSIL/CIN2, 3 and AIS or adenocarcinoma in follow-up biopsies and high HR-HPV viral load. In addition, there were significant differences (P = 0.012) in specificity, but no significant differences were found in sensitivity (P = 0.604), positive and negative predictive value (P = 0.066 and 0.264) between p16 immunoexpression and HR-HPV viral load. Conclusions. Pathogenic activity of HR-HPV was indicated by p16 expression on smears and tissue sections, which appears to be a better strategy than HR-HPV viral load test for the detection of clinically insignificant lesions from AGC-categorized Pap smears.

KW - Atypical glandular cells

KW - Human papillomavirus

KW - Immunocytochemistry

KW - p16

UR - http://www.scopus.com/inward/record.url?scp=28044442252&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28044442252&partnerID=8YFLogxK

U2 - 10.1016/j.ygyno.2005.06.060

DO - 10.1016/j.ygyno.2005.06.060

M3 - Article

C2 - 16139346

AN - SCOPUS:28044442252

VL - 99

SP - 578

EP - 584

JO - Gynecologic Oncology

JF - Gynecologic Oncology

SN - 0090-8258

IS - 3

ER -