Which test is a better strategy to determine the outcome of atypical glandular cell-categorized Pap smears? Immunocytochemical p16INK4A expression or human papillomavirus test - A retrospective cohort study

Su Feng Chen, Shih Fang Yang, Tang Yuan Chu, Hung Cheng Lai, Ya Wen Lin, Chien Yu Bai, Shin Nieh

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective. This study was to correlate high-risk human papillomavirus (HR-HPV) viral load to p16INK4A (p16) expression in atypical glandular cell (AGC)-categorized Pap smears with follow-up biopsies for elucidating their relationships. Methods. We enrolled 36 AGC-categorized Pap smears with subsequent follow-up biopsies. HR-HPV viral load was determined by Hybrid Capture II assay in each AGC-diagnosed Pap smear. Both smears and biopsies were immunostained with a primary anti-p16 antibody, clone E6H4. Correlations between HR-HPV viral load in each AGC-diagnosed Pap smear and p16 expression of smears with follow-up biopsies were performed. Results. Comparative analysis of two tests disclosed both consistencies and discrepancies. There were significant differences (P = 0.02) between negative or weak p16 expression of Pap smears with the presence of reactive lesion or LSILs/CIN1s in follow-up biopsies and negative HR-HPV viral load. However, no significant difference (P = 0.317) was found between p16 expression of Pap smears with the presence of HSIL/CIN2, 3 and AIS or adenocarcinoma in follow-up biopsies and high HR-HPV viral load. In addition, there were significant differences (P = 0.012) in specificity, but no significant differences were found in sensitivity (P = 0.604), positive and negative predictive value (P = 0.066 and 0.264) between p16 immunoexpression and HR-HPV viral load. Conclusions. Pathogenic activity of HR-HPV was indicated by p16 expression on smears and tissue sections, which appears to be a better strategy than HR-HPV viral load test for the detection of clinically insignificant lesions from AGC-categorized Pap smears.

Original languageEnglish
Pages (from-to)578-584
Number of pages7
JournalGynecologic Oncology
Volume99
Issue number3
DOIs
Publication statusPublished - Dec 2005
Externally publishedYes

Keywords

  • Atypical glandular cells
  • Human papillomavirus
  • Immunocytochemistry
  • p16

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology

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