Water-Soluble Fullerenol C60(OH)36 toward Effective Anti-Air Pollution Induced by Urban Particulate Matter in HaCaT Cell

Chiang Wen Lee, Miao Ching Chi, Kuo Ti Peng, Yao Chang Chiang, Lee Fen Hsu, Yi Ling Yan, Hsing Yen Li, Ming Chun Chen, I. Ta Lee, Chian Hui Lai

Research output: Contribution to journalArticle

Abstract

Particulate matter (PM), a widespread air pollutant, consists of a complex mixture of solid and liquid particles suspended in air. Many diseases have been linked to PM exposure, which induces an imbalance in reactive oxygen species (ROS) generated in cells, and might result in skin diseases (such as aging and atopic dermatitis). New techniques involving nanomedicine and nano-delivery systems are being rapidly developed in the medicinal field. Fullerene, a kind of nanomaterial, acts as a super radical scavenger. Lower water solubility levels limit the bio-applications of fullerene. Hence, to improve the water solubility of fullerene, while retaining its radical scavenger functions, a fullerene derivative, fullerenol C60(OH)36, was synthesized, to examine its biofunctions in PM-exposed human keratinocyte (HaCaT) cells. The PM-induced increase in ROS levels and expression of phosphorylated mitogen-activated protein kinase and Akt could be inhibited via fullerenol pre-treatment. Furthermore, the expression of inflammation-related proteins, cyclooxygenase-2, heme oxygenase-1, and prostaglandin E2 was also suppressed. Fullerenol could preserve the impaired state of skin barrier proteins (filaggrin, involucrin, repetin, and loricrin), which was attributable to PM exposure. These results suggest that fullerenol could act against PM-induced cytotoxicity via ROS scavenging and anti-inflammatory mechanisms, and the maintenance of expression of barrier proteins, and is a potential candidate compound for the treatment of skin diseases.

Original languageEnglish
JournalInternational Journal of Molecular Sciences
Volume20
Issue number17
DOIs
Publication statusPublished - Aug 30 2019

Fingerprint

air pollution
Particulate Matter
Air Pollution
Fullerenes
Air pollution
particulates
Proteins
Skin
fullerenes
Water
cells
water
Oxygen
proteins
Reactive Oxygen Species
Solubility
Medical nanotechnology
Skin Diseases
Scavenging
oxygen

Keywords

  • anti-inflammation
  • antioxidant
  • fullerenol
  • human keratinocyte cell
  • particulate matter
  • reactive oxygen species

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Water-Soluble Fullerenol C60(OH)36 toward Effective Anti-Air Pollution Induced by Urban Particulate Matter in HaCaT Cell. / Lee, Chiang Wen; Chi, Miao Ching; Peng, Kuo Ti; Chiang, Yao Chang; Hsu, Lee Fen; Yan, Yi Ling; Li, Hsing Yen; Chen, Ming Chun; Lee, I. Ta; Lai, Chian Hui.

In: International Journal of Molecular Sciences, Vol. 20, No. 17, 30.08.2019.

Research output: Contribution to journalArticle

Lee, Chiang Wen ; Chi, Miao Ching ; Peng, Kuo Ti ; Chiang, Yao Chang ; Hsu, Lee Fen ; Yan, Yi Ling ; Li, Hsing Yen ; Chen, Ming Chun ; Lee, I. Ta ; Lai, Chian Hui. / Water-Soluble Fullerenol C60(OH)36 toward Effective Anti-Air Pollution Induced by Urban Particulate Matter in HaCaT Cell. In: International Journal of Molecular Sciences. 2019 ; Vol. 20, No. 17.
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abstract = "Particulate matter (PM), a widespread air pollutant, consists of a complex mixture of solid and liquid particles suspended in air. Many diseases have been linked to PM exposure, which induces an imbalance in reactive oxygen species (ROS) generated in cells, and might result in skin diseases (such as aging and atopic dermatitis). New techniques involving nanomedicine and nano-delivery systems are being rapidly developed in the medicinal field. Fullerene, a kind of nanomaterial, acts as a super radical scavenger. Lower water solubility levels limit the bio-applications of fullerene. Hence, to improve the water solubility of fullerene, while retaining its radical scavenger functions, a fullerene derivative, fullerenol C60(OH)36, was synthesized, to examine its biofunctions in PM-exposed human keratinocyte (HaCaT) cells. The PM-induced increase in ROS levels and expression of phosphorylated mitogen-activated protein kinase and Akt could be inhibited via fullerenol pre-treatment. Furthermore, the expression of inflammation-related proteins, cyclooxygenase-2, heme oxygenase-1, and prostaglandin E2 was also suppressed. Fullerenol could preserve the impaired state of skin barrier proteins (filaggrin, involucrin, repetin, and loricrin), which was attributable to PM exposure. These results suggest that fullerenol could act against PM-induced cytotoxicity via ROS scavenging and anti-inflammatory mechanisms, and the maintenance of expression of barrier proteins, and is a potential candidate compound for the treatment of skin diseases.",
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AU - Chiang, Yao Chang

AU - Hsu, Lee Fen

AU - Yan, Yi Ling

AU - Li, Hsing Yen

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AU - Lee, I. Ta

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