Abstract

The objective of this study is to investigate the synergistic effects of acid etching and metal-ion chelation in dental smear layer removal using wasted Ganoderma tsugae derived chitosans. The wasted Ganoderma tsugae fruiting body was used to prepare both acid-soluble fungal chitosan (FCS) and alkali-soluble polysaccharide (ASP). To explore the effective irrigant concentration for smear layer removal, a chelating effect on ferrous ions was conducted. Specimens of various concentrations of EDTA, citric acid, and polysaccharide solutions were reacted with FerroZineTM then the absorbance was examined at 562 nm by aUV-visible spectrophotometer to calculate their metal chelating capability. Twenty extracted premolars were instrumented and individually soaked in the solutions of 15 wt% EDTA, 10 wt% citric acid, 0.04 wt% ASP, 0.04 wt% FCS, and normal saline were randomly divided into five groups (N=4). Next, each tooth was cleaved longitudinally and examined by scanning electron microscopy (SEM) to assay the effectiveness of smear layer removal. The chelating capability for EDTA, FCS, and ASP showed no significant difference over the concentration of 0.04 wt% (p > 0.05). The SEM results showed that 0.04 wt% FCS solution was effective in smear layer removal along the canal wall. These results indicated that Ganoderma tsuage derived FCS in acid solutions could be a potential alternative as a root canal irrigant solution due to its synergistic effect.

Original languageEnglish
Article number1795
JournalPolymers
Volume11
Issue number11
DOIs
Publication statusPublished - Nov 1 2019

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Chitosan
Polysaccharides
Chelation
Ethylenediaminetetraacetic acid
Alkalies
Edetic Acid
Citric acid
Canals
Citric Acid
Acids
Root Canal Irrigants
Scanning electron microscopy
Spectrophotometers
Metal ions
Etching
Assays
Metals
Ions

Keywords

  • Chelating effect
  • EDTA
  • Polysaccharide
  • Smear layer removal

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

Cite this

Wasted Ganoderma tsugae derived chitosans for smear layer removal in endodontic treatment. / Huang, Sheng Tung; Teng, Nai Chia; Wang, Hsin Hui; Hsieh, Sung Chih; Yang, Jen Chang.

In: Polymers, Vol. 11, No. 11, 1795, 01.11.2019.

Research output: Contribution to journalArticle

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