VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cell surface

Satoshi Kawaguchi, Kokichi Kikuchi, Seiichi Ishii, Yoshikazu Takada, Seiichi Kobayashi, Toshimitsu Uede

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

To elucidate the role of VLA-4 (α4β1 integrin) in tumor metastasis, we have transfected cDNA coding α4 subunit into human fibrosarcoma (HT1080) cells. VLA-4-overexpressing HT-VC1 cells exhibited increased ability to interact with known ligands for VLA-4, such as CS1 peptide and VCAM-1 (vascular cell adhesion molecule-1). In addition, the in vitro invasive ability of HT-VC1 cells was augmented and the mRNA for type IV collagenase was increased in HT-VC1 cells. The induction of VCAM-1 molecules on lung endothelial cells of nude mice by tumor necrosis factor-α treatment resulted in augmentation of in vivo HT-VC1 cell adhesion to the lung endothelial cells. Thus, the VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cells, that is important for hematogenous metastasis.

Original languageEnglish
Pages (from-to)1304-1316
Number of pages13
JournalJapanese Journal of Cancer Research
Volume83
Issue number12
Publication statusPublished - Dec 1992
Externally publishedYes

Fingerprint

Integrin alpha4beta1
Vascular Cell Adhesion Molecule-1
Adhesives
Endothelial Cells
Neoplasms
Neoplasm Metastasis
Lung
Fibrosarcoma
Collagenases
Nude Mice
Cell Adhesion
Integrins
Complementary DNA
Tumor Necrosis Factor-alpha
Ligands
Messenger RNA

Keywords

  • cDNA transfection
  • Human sarcoma cell
  • Integrin
  • Metastasis
  • VLA-4

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Kawaguchi, S., Kikuchi, K., Ishii, S., Takada, Y., Kobayashi, S., & Uede, T. (1992). VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cell surface. Japanese Journal of Cancer Research, 83(12), 1304-1316.

VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cell surface. / Kawaguchi, Satoshi; Kikuchi, Kokichi; Ishii, Seiichi; Takada, Yoshikazu; Kobayashi, Seiichi; Uede, Toshimitsu.

In: Japanese Journal of Cancer Research, Vol. 83, No. 12, 12.1992, p. 1304-1316.

Research output: Contribution to journalArticle

Kawaguchi, S, Kikuchi, K, Ishii, S, Takada, Y, Kobayashi, S & Uede, T 1992, 'VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cell surface', Japanese Journal of Cancer Research, vol. 83, no. 12, pp. 1304-1316.
Kawaguchi, Satoshi ; Kikuchi, Kokichi ; Ishii, Seiichi ; Takada, Yoshikazu ; Kobayashi, Seiichi ; Uede, Toshimitsu. / VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cell surface. In: Japanese Journal of Cancer Research. 1992 ; Vol. 83, No. 12. pp. 1304-1316.
@article{208823be293049fa8fbceae1d1b100c1,
title = "VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cell surface",
abstract = "To elucidate the role of VLA-4 (α4β1 integrin) in tumor metastasis, we have transfected cDNA coding α4 subunit into human fibrosarcoma (HT1080) cells. VLA-4-overexpressing HT-VC1 cells exhibited increased ability to interact with known ligands for VLA-4, such as CS1 peptide and VCAM-1 (vascular cell adhesion molecule-1). In addition, the in vitro invasive ability of HT-VC1 cells was augmented and the mRNA for type IV collagenase was increased in HT-VC1 cells. The induction of VCAM-1 molecules on lung endothelial cells of nude mice by tumor necrosis factor-α treatment resulted in augmentation of in vivo HT-VC1 cell adhesion to the lung endothelial cells. Thus, the VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cells, that is important for hematogenous metastasis.",
keywords = "cDNA transfection, Human sarcoma cell, Integrin, Metastasis, VLA-4",
author = "Satoshi Kawaguchi and Kokichi Kikuchi and Seiichi Ishii and Yoshikazu Takada and Seiichi Kobayashi and Toshimitsu Uede",
year = "1992",
month = "12",
language = "English",
volume = "83",
pages = "1304--1316",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "12",

}

TY - JOUR

T1 - VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cell surface

AU - Kawaguchi, Satoshi

AU - Kikuchi, Kokichi

AU - Ishii, Seiichi

AU - Takada, Yoshikazu

AU - Kobayashi, Seiichi

AU - Uede, Toshimitsu

PY - 1992/12

Y1 - 1992/12

N2 - To elucidate the role of VLA-4 (α4β1 integrin) in tumor metastasis, we have transfected cDNA coding α4 subunit into human fibrosarcoma (HT1080) cells. VLA-4-overexpressing HT-VC1 cells exhibited increased ability to interact with known ligands for VLA-4, such as CS1 peptide and VCAM-1 (vascular cell adhesion molecule-1). In addition, the in vitro invasive ability of HT-VC1 cells was augmented and the mRNA for type IV collagenase was increased in HT-VC1 cells. The induction of VCAM-1 molecules on lung endothelial cells of nude mice by tumor necrosis factor-α treatment resulted in augmentation of in vivo HT-VC1 cell adhesion to the lung endothelial cells. Thus, the VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cells, that is important for hematogenous metastasis.

AB - To elucidate the role of VLA-4 (α4β1 integrin) in tumor metastasis, we have transfected cDNA coding α4 subunit into human fibrosarcoma (HT1080) cells. VLA-4-overexpressing HT-VC1 cells exhibited increased ability to interact with known ligands for VLA-4, such as CS1 peptide and VCAM-1 (vascular cell adhesion molecule-1). In addition, the in vitro invasive ability of HT-VC1 cells was augmented and the mRNA for type IV collagenase was increased in HT-VC1 cells. The induction of VCAM-1 molecules on lung endothelial cells of nude mice by tumor necrosis factor-α treatment resulted in augmentation of in vivo HT-VC1 cell adhesion to the lung endothelial cells. Thus, the VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cells, that is important for hematogenous metastasis.

KW - cDNA transfection

KW - Human sarcoma cell

KW - Integrin

KW - Metastasis

KW - VLA-4

UR - http://www.scopus.com/inward/record.url?scp=0027064476&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027064476&partnerID=8YFLogxK

M3 - Article

VL - 83

SP - 1304

EP - 1316

JO - Cancer Science

JF - Cancer Science

SN - 1347-9032

IS - 12

ER -