Abstract
To elucidate the role of VLA-4 (α4β1 integrin) in tumor metastasis, we have transfected cDNA coding α4 subunit into human fibrosarcoma (HT1080) cells. VLA-4-overexpressing HT-VC1 cells exhibited increased ability to interact with known ligands for VLA-4, such as CS1 peptide and VCAM-1 (vascular cell adhesion molecule-1). In addition, the in vitro invasive ability of HT-VC1 cells was augmented and the mRNA for type IV collagenase was increased in HT-VC1 cells. The induction of VCAM-1 molecules on lung endothelial cells of nude mice by tumor necrosis factor-α treatment resulted in augmentation of in vivo HT-VC1 cell adhesion to the lung endothelial cells. Thus, the VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cells, that is important for hematogenous metastasis.
Original language | English |
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Pages (from-to) | 1304-1316 |
Number of pages | 13 |
Journal | Japanese Journal of Cancer Research |
Volume | 83 |
Issue number | 12 |
Publication status | Published - Dec 1992 |
Externally published | Yes |
Keywords
- cDNA transfection
- Human sarcoma cell
- Integrin
- Metastasis
- VLA-4
ASJC Scopus subject areas
- Oncology
- Cancer Research