VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cell surface

Satoshi Kawaguchi, Kokichi Kikuchi, Seiichi Ishii, Yoshikazu Takada, Seiichi Kobayashi, Toshimitsu Uede

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

To elucidate the role of VLA-4 (α4β1 integrin) in tumor metastasis, we have transfected cDNA coding α4 subunit into human fibrosarcoma (HT1080) cells. VLA-4-overexpressing HT-VC1 cells exhibited increased ability to interact with known ligands for VLA-4, such as CS1 peptide and VCAM-1 (vascular cell adhesion molecule-1). In addition, the in vitro invasive ability of HT-VC1 cells was augmented and the mRNA for type IV collagenase was increased in HT-VC1 cells. The induction of VCAM-1 molecules on lung endothelial cells of nude mice by tumor necrosis factor-α treatment resulted in augmentation of in vivo HT-VC1 cell adhesion to the lung endothelial cells. Thus, the VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cells, that is important for hematogenous metastasis.

Original languageEnglish
Pages (from-to)1304-1316
Number of pages13
JournalJapanese Journal of Cancer Research
Volume83
Issue number12
Publication statusPublished - Dec 1992
Externally publishedYes

Keywords

  • cDNA transfection
  • Human sarcoma cell
  • Integrin
  • Metastasis
  • VLA-4

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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