Abstract
To elucidate the role of VLA-4 (α4β1 integrin) in tumor metastasis, we have transfected cDNA coding α4 subunit into human fibrosarcoma (HT1080) cells. VLA-4-overexpressing HT-VC1 cells exhibited increased ability to interact with known ligands for VLA-4, such as CS1 peptide and VCAM-1 (vascular cell adhesion molecule-1). In addition, the in vitro invasive ability of HT-VC1 cells was augmented and the mRNA for type IV collagenase was increased in HT-VC1 cells. The induction of VCAM-1 molecules on lung endothelial cells of nude mice by tumor necrosis factor-α treatment resulted in augmentation of in vivo HT-VC1 cell adhesion to the lung endothelial cells. Thus, the VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cells, that is important for hematogenous metastasis.
| Original language | English |
|---|---|
| Pages (from-to) | 1304-1316 |
| Number of pages | 13 |
| Journal | Japanese Journal of Cancer Research |
| Volume | 83 |
| Issue number | 12 |
| Publication status | Published - Dec 1992 |
| Externally published | Yes |
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Keywords
- cDNA transfection
- Human sarcoma cell
- Integrin
- Metastasis
- VLA-4
ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cell surface. / Kawaguchi, Satoshi; Kikuchi, Kokichi; Ishii, Seiichi; Takada, Yoshikazu; Kobayashi, Seiichi; Uede, Toshimitsu.
In: Japanese Journal of Cancer Research, Vol. 83, No. 12, 12.1992, p. 1304-1316.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cell surface
AU - Kawaguchi, Satoshi
AU - Kikuchi, Kokichi
AU - Ishii, Seiichi
AU - Takada, Yoshikazu
AU - Kobayashi, Seiichi
AU - Uede, Toshimitsu
PY - 1992/12
Y1 - 1992/12
N2 - To elucidate the role of VLA-4 (α4β1 integrin) in tumor metastasis, we have transfected cDNA coding α4 subunit into human fibrosarcoma (HT1080) cells. VLA-4-overexpressing HT-VC1 cells exhibited increased ability to interact with known ligands for VLA-4, such as CS1 peptide and VCAM-1 (vascular cell adhesion molecule-1). In addition, the in vitro invasive ability of HT-VC1 cells was augmented and the mRNA for type IV collagenase was increased in HT-VC1 cells. The induction of VCAM-1 molecules on lung endothelial cells of nude mice by tumor necrosis factor-α treatment resulted in augmentation of in vivo HT-VC1 cell adhesion to the lung endothelial cells. Thus, the VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cells, that is important for hematogenous metastasis.
AB - To elucidate the role of VLA-4 (α4β1 integrin) in tumor metastasis, we have transfected cDNA coding α4 subunit into human fibrosarcoma (HT1080) cells. VLA-4-overexpressing HT-VC1 cells exhibited increased ability to interact with known ligands for VLA-4, such as CS1 peptide and VCAM-1 (vascular cell adhesion molecule-1). In addition, the in vitro invasive ability of HT-VC1 cells was augmented and the mRNA for type IV collagenase was increased in HT-VC1 cells. The induction of VCAM-1 molecules on lung endothelial cells of nude mice by tumor necrosis factor-α treatment resulted in augmentation of in vivo HT-VC1 cell adhesion to the lung endothelial cells. Thus, the VLA-4 molecules on tumor cells initiate an adhesive interaction with VCAM-1 molecules on endothelial cells, that is important for hematogenous metastasis.
KW - cDNA transfection
KW - Human sarcoma cell
KW - Integrin
KW - Metastasis
KW - VLA-4
UR - http://www.scopus.com/inward/record.url?scp=0027064476&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027064476&partnerID=8YFLogxK
M3 - Article
VL - 83
SP - 1304
EP - 1316
JO - Cancer Science
JF - Cancer Science
SN - 1347-9032
IS - 12
ER -