Vitis thunbergii var. taiwaniana Extracts and Purified Compounds Ameliorate Obesity in High-Fat Diet-Induced Obese Mice

Shyr Yi Lin, Guan Cheng Huang, Ying Ying Hsieh, Yin Shiou Lin, Chuan Hsiao Han, Chi Luan Wen, Chi I. Chang, Wen Chi Hou

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The increasing prevalence of obesity continues to gain more attention worldwide. In this study, diet-induced obese mice were used to evaluate the antiobesity effects of extracts, fractions, and purified compounds from Vitis thunbergii var. taiwaniana (VTT). The C57BL/6J mice were fed a 5-week high-fat diet (HF) concurrently with ethanol extracts (Et-ext, 80 mg/kg) from roots (R), stems (S), and leaves (L) by oral gavage daily. Only R-Et-ext interventions showed significant weight reduction in mice compared with those in the HF group; however, mouse plasma contents of total cholesterols (TC), total triglycerides (TG) and low-density lipoproteins (LDL) of all three Et-ext intervened groups showed significant reductions compared with those in the HF group. Furthermore, intervention with the ethyl acetate-partitioned fraction (EA-fra, 60 mg/kg) from R-Et-ext but not the n-butanol-partitioned fraction or water fraction from R-Et-ext showed significant weight reduction in mice compared with those in the HF group. The same molecular weights of three resveratrol tetramers, (+)-hopeaphenol, (+)-vitisin A, and (-)-vitisin B, were isolated from the EA-fra of VTT-R. The (+)-vitisin A and fenofibrate (25 mg/kg) but not the (+)-hopeaphenol and (-)-vitisin B interventions showed significant weight reduction in mice compared with those in the HF group. The total feed intake among the HF groups with or without interventions showed no significant differences. The mouse plasma contents of TC, TG, LDL, free fatty acid, and plasma lipase activity of the three resveratrol tetramer-intervened groups showed reductions in the mice compared with those in the HF group. It was proposed that the lipase inhibitory activities of VTT extracts and purified resveratrol tetramers might contribute in part to the antiobesity effect, and these results suggested that VTT may be developed as functional food for achieving antiobesity objectives and requires further investigation.

Original languageEnglish
Pages (from-to)9286-9294
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number42
DOIs
Publication statusPublished - Oct 28 2015

Fingerprint

Obese Mice
Vitis
High Fat Diet
high fat diet
Nutrition
obesity
Obesity
Fats
mice
extracts
resveratrol
Weight Loss
weight loss
Lipase
low density lipoprotein
Plasmas
Fenofibrate
triacylglycerols
Cholesterol
cholesterol

Keywords

  • (+)-vitisin A
  • high-fat diet (HF)
  • lipase
  • obesity
  • Vitis thunbergii var. taiwaniana (VTT)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Vitis thunbergii var. taiwaniana Extracts and Purified Compounds Ameliorate Obesity in High-Fat Diet-Induced Obese Mice. / Lin, Shyr Yi; Huang, Guan Cheng; Hsieh, Ying Ying; Lin, Yin Shiou; Han, Chuan Hsiao; Wen, Chi Luan; Chang, Chi I.; Hou, Wen Chi.

In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 42, 28.10.2015, p. 9286-9294.

Research output: Contribution to journalArticle

Lin, Shyr Yi ; Huang, Guan Cheng ; Hsieh, Ying Ying ; Lin, Yin Shiou ; Han, Chuan Hsiao ; Wen, Chi Luan ; Chang, Chi I. ; Hou, Wen Chi. / Vitis thunbergii var. taiwaniana Extracts and Purified Compounds Ameliorate Obesity in High-Fat Diet-Induced Obese Mice. In: Journal of Agricultural and Food Chemistry. 2015 ; Vol. 63, No. 42. pp. 9286-9294.
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abstract = "The increasing prevalence of obesity continues to gain more attention worldwide. In this study, diet-induced obese mice were used to evaluate the antiobesity effects of extracts, fractions, and purified compounds from Vitis thunbergii var. taiwaniana (VTT). The C57BL/6J mice were fed a 5-week high-fat diet (HF) concurrently with ethanol extracts (Et-ext, 80 mg/kg) from roots (R), stems (S), and leaves (L) by oral gavage daily. Only R-Et-ext interventions showed significant weight reduction in mice compared with those in the HF group; however, mouse plasma contents of total cholesterols (TC), total triglycerides (TG) and low-density lipoproteins (LDL) of all three Et-ext intervened groups showed significant reductions compared with those in the HF group. Furthermore, intervention with the ethyl acetate-partitioned fraction (EA-fra, 60 mg/kg) from R-Et-ext but not the n-butanol-partitioned fraction or water fraction from R-Et-ext showed significant weight reduction in mice compared with those in the HF group. The same molecular weights of three resveratrol tetramers, (+)-hopeaphenol, (+)-vitisin A, and (-)-vitisin B, were isolated from the EA-fra of VTT-R. The (+)-vitisin A and fenofibrate (25 mg/kg) but not the (+)-hopeaphenol and (-)-vitisin B interventions showed significant weight reduction in mice compared with those in the HF group. The total feed intake among the HF groups with or without interventions showed no significant differences. The mouse plasma contents of TC, TG, LDL, free fatty acid, and plasma lipase activity of the three resveratrol tetramer-intervened groups showed reductions in the mice compared with those in the HF group. It was proposed that the lipase inhibitory activities of VTT extracts and purified resveratrol tetramers might contribute in part to the antiobesity effect, and these results suggested that VTT may be developed as functional food for achieving antiobesity objectives and requires further investigation.",
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