Vitamin D3 attenuates cortical infarction induced by middle cerebral arterial ligation in rats

Y. Wang, Y. H. Chiang, T. P. Su, T. Hayashi, M. Morales, B. J. Hoffer, S. Z. Lin

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

We have previously reported that intracerebral administration of glial cell line derived neurotrophic factor (GDNF) reduces the extent of middle cerebral arterial (MCA) ligation-induced cortical infarction in rats. Recent studies have shown that application of 1,25 dihydroxyvitamin D3 (D3) enhances GDNF mRNA expression in vitro. The purpose of the present study was to investigate if administration of D3 in vivo will protect against ischemic brain injury. Adult male Sprague-Dawley rats were injected daily with D3 or with saline for four or eight days. Animals received a 90-min right MCA ligation on the 4(th) or 8(th) day after anesthesia with chloral hydrate. Animals were sacrificed for tri-phenyl-tetrazolium chloride (TTC) staining 24 h after the onset of reperfusion. A subset of animals receiving eight days of D3 or saline treatment were used for blood gas and cerebral GDNF protein level analysis. We found that pretreatment with D3 for four days did not attenuate the ischemic injury. However, animals receiving eight days of D3 injections showed a significant reduction in the amount of infarction in the cortex. Eight day D3 treatment did not alter blood gases or blood pressure; however, it did increase calcium levels. Pretreatment with D3 significantly increased GDNF levels in the cortex. In conclusion, our data indicate that D3 reduces ischemia- induced brain damage and supports the hypothesis that this effect may be through the up-regulation of GDNF mechanisms in cortex. Copyright (C) 1999.

Original languageEnglish
Pages (from-to)873-880
Number of pages8
JournalNeuropharmacology
Volume39
Issue number5
DOIs
Publication statusPublished - Apr 2000
Externally publishedYes

Fingerprint

Glial Cell Line-Derived Neurotrophic Factor
Cholecalciferol
Infarction
Ligation
Gases
Chloral Hydrate
Calcitriol
Brain Ischemia
Brain Injuries
Reperfusion
Sprague Dawley Rats
Chlorides
Up-Regulation
Anesthesia
Staining and Labeling
Blood Pressure
Calcium
Messenger RNA
Injections
Wounds and Injuries

Keywords

  • Glial cell line-derived neurotrophic factor
  • Infarction
  • Ischemia
  • Neuroprotection
  • Vitamin D

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

Vitamin D3 attenuates cortical infarction induced by middle cerebral arterial ligation in rats. / Wang, Y.; Chiang, Y. H.; Su, T. P.; Hayashi, T.; Morales, M.; Hoffer, B. J.; Lin, S. Z.

In: Neuropharmacology, Vol. 39, No. 5, 04.2000, p. 873-880.

Research output: Contribution to journalArticle

Wang, Y. ; Chiang, Y. H. ; Su, T. P. ; Hayashi, T. ; Morales, M. ; Hoffer, B. J. ; Lin, S. Z. / Vitamin D3 attenuates cortical infarction induced by middle cerebral arterial ligation in rats. In: Neuropharmacology. 2000 ; Vol. 39, No. 5. pp. 873-880.
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