Vitamin C protects against lysophosphatidylcholine-induced expression of monocyte chemoattractant protein-1 in cultured human umbilical vein endothelial cells

Ju P. Pan, Tsai M. Cheng, Shiu Chin Chou, Shiau Ting Lai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background and Purpose: It is well documented that oxidized low-density lipoproteins (LDLs) can stimulate human vascular endothelial cells to produce monocyte chemoattractant protein-1 (MGP-1). Vitamin C is known to be an important antioxidant for vasodilatation. The purpose of this study was to determine whether pretreatment with vitamin C could protect against oxidized-LDL-induced expression of MCP-1 in cultured human umbilical vein endothelial cells (HUVECs). Methods: Cultured HUVECs were used for desired experiments before passage 4. Lysophosphatidylcholine (lysoPC), an oxidized component of LDL, was designated as the stimulator for MCP-1 synthesis from cultured HUVECs. MCP-1 concentrations in the cultured media were determined by enzyme-linked immunosorbent assay. MCP-1 RNA was evaluated by a semi-quantitative reverse transcriptase-polymerase chain reaction. Results: HUVECs secreted MCP-1 within 30 minutes after exposure to 50 μM lysoPC. Compared with samples treated with lysoPC alone, pretreatment with vitamin C in concentrations of 50, 100, 150, and 200 μM, reduced levels of MCP-1 in the culture medium by 44%, 51%, 60%, and 67%, respectively, while levels of MCP-1 mRNA decreased by 15%, 18%, 80%, and 82%, respectively. Conclusions: Our findings imply that pretreatment with vitamin C can suppress lysoPC-induced expression and secretion of MCP-1 in cultured HUVECs. Therefore, vitamin C is protective against lysoPC-mediated inflammatory insults to the vascular endothelium in vitro.

Original languageEnglish
Pages (from-to)151-157
Number of pages7
JournalJournal of the Formosan Medical Association = Taiwan yi zhi
Volume102
Issue number3
Publication statusPublished - Mar 1 2003
Externally publishedYes

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Keywords

  • Ascorbic acid
  • Cell culture
  • Lysophosphatidylcholines
  • Monocyte chemoattractant protein-1
  • Protein kinase C

ASJC Scopus subject areas

  • Medicine(all)

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