Vitamin C protects against lysophosphatidylcholine-induced expression of monocyte chemoattractant protein-1 in cultured human umbilical vein endothelial cells

Ju P. Pan, Tsai M. Cheng, Shiu Chin Chou, Shiau Ting Lai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background and Purpose: It is well documented that oxidized low-density lipoproteins (LDLs) can stimulate human vascular endothelial cells to produce monocyte chemoattractant protein-1 (MGP-1). Vitamin C is known to be an important antioxidant for vasodilatation. The purpose of this study was to determine whether pretreatment with vitamin C could protect against oxidized-LDL-induced expression of MCP-1 in cultured human umbilical vein endothelial cells (HUVECs). Methods: Cultured HUVECs were used for desired experiments before passage 4. Lysophosphatidylcholine (lysoPC), an oxidized component of LDL, was designated as the stimulator for MCP-1 synthesis from cultured HUVECs. MCP-1 concentrations in the cultured media were determined by enzyme-linked immunosorbent assay. MCP-1 RNA was evaluated by a semi-quantitative reverse transcriptase-polymerase chain reaction. Results: HUVECs secreted MCP-1 within 30 minutes after exposure to 50 μM lysoPC. Compared with samples treated with lysoPC alone, pretreatment with vitamin C in concentrations of 50, 100, 150, and 200 μM, reduced levels of MCP-1 in the culture medium by 44%, 51%, 60%, and 67%, respectively, while levels of MCP-1 mRNA decreased by 15%, 18%, 80%, and 82%, respectively. Conclusions: Our findings imply that pretreatment with vitamin C can suppress lysoPC-induced expression and secretion of MCP-1 in cultured HUVECs. Therefore, vitamin C is protective against lysoPC-mediated inflammatory insults to the vascular endothelium in vitro.

Original languageEnglish
Pages (from-to)151-157
Number of pages7
JournalJournal of the Formosan Medical Association = Taiwan yi zhi
Volume102
Issue number3
Publication statusPublished - Mar 1 2003
Externally publishedYes

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Lysophosphatidylcholines
Chemokine CCL2
Human Umbilical Vein Endothelial Cells
Ascorbic Acid
Vascular Endothelium
Reverse Transcriptase Polymerase Chain Reaction
Vasodilation
Culture Media
Endothelial Cells
Antioxidants
Enzyme-Linked Immunosorbent Assay
RNA
Messenger RNA
oxidized low density lipoprotein

Keywords

  • Ascorbic acid
  • Cell culture
  • Lysophosphatidylcholines
  • Monocyte chemoattractant protein-1
  • Protein kinase C

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{89444c41eda14c6cadebb1b6fb27445e,
title = "Vitamin C protects against lysophosphatidylcholine-induced expression of monocyte chemoattractant protein-1 in cultured human umbilical vein endothelial cells",
abstract = "Background and Purpose: It is well documented that oxidized low-density lipoproteins (LDLs) can stimulate human vascular endothelial cells to produce monocyte chemoattractant protein-1 (MGP-1). Vitamin C is known to be an important antioxidant for vasodilatation. The purpose of this study was to determine whether pretreatment with vitamin C could protect against oxidized-LDL-induced expression of MCP-1 in cultured human umbilical vein endothelial cells (HUVECs). Methods: Cultured HUVECs were used for desired experiments before passage 4. Lysophosphatidylcholine (lysoPC), an oxidized component of LDL, was designated as the stimulator for MCP-1 synthesis from cultured HUVECs. MCP-1 concentrations in the cultured media were determined by enzyme-linked immunosorbent assay. MCP-1 RNA was evaluated by a semi-quantitative reverse transcriptase-polymerase chain reaction. Results: HUVECs secreted MCP-1 within 30 minutes after exposure to 50 μM lysoPC. Compared with samples treated with lysoPC alone, pretreatment with vitamin C in concentrations of 50, 100, 150, and 200 μM, reduced levels of MCP-1 in the culture medium by 44{\%}, 51{\%}, 60{\%}, and 67{\%}, respectively, while levels of MCP-1 mRNA decreased by 15{\%}, 18{\%}, 80{\%}, and 82{\%}, respectively. Conclusions: Our findings imply that pretreatment with vitamin C can suppress lysoPC-induced expression and secretion of MCP-1 in cultured HUVECs. Therefore, vitamin C is protective against lysoPC-mediated inflammatory insults to the vascular endothelium in vitro.",
keywords = "Ascorbic acid, Cell culture, Lysophosphatidylcholines, Monocyte chemoattractant protein-1, Protein kinase C",
author = "Pan, {Ju P.} and Cheng, {Tsai M.} and Chou, {Shiu Chin} and Lai, {Shiau Ting}",
year = "2003",
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language = "English",
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pages = "151--157",
journal = "Journal of the Formosan Medical Association",
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TY - JOUR

T1 - Vitamin C protects against lysophosphatidylcholine-induced expression of monocyte chemoattractant protein-1 in cultured human umbilical vein endothelial cells

AU - Pan, Ju P.

AU - Cheng, Tsai M.

AU - Chou, Shiu Chin

AU - Lai, Shiau Ting

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Background and Purpose: It is well documented that oxidized low-density lipoproteins (LDLs) can stimulate human vascular endothelial cells to produce monocyte chemoattractant protein-1 (MGP-1). Vitamin C is known to be an important antioxidant for vasodilatation. The purpose of this study was to determine whether pretreatment with vitamin C could protect against oxidized-LDL-induced expression of MCP-1 in cultured human umbilical vein endothelial cells (HUVECs). Methods: Cultured HUVECs were used for desired experiments before passage 4. Lysophosphatidylcholine (lysoPC), an oxidized component of LDL, was designated as the stimulator for MCP-1 synthesis from cultured HUVECs. MCP-1 concentrations in the cultured media were determined by enzyme-linked immunosorbent assay. MCP-1 RNA was evaluated by a semi-quantitative reverse transcriptase-polymerase chain reaction. Results: HUVECs secreted MCP-1 within 30 minutes after exposure to 50 μM lysoPC. Compared with samples treated with lysoPC alone, pretreatment with vitamin C in concentrations of 50, 100, 150, and 200 μM, reduced levels of MCP-1 in the culture medium by 44%, 51%, 60%, and 67%, respectively, while levels of MCP-1 mRNA decreased by 15%, 18%, 80%, and 82%, respectively. Conclusions: Our findings imply that pretreatment with vitamin C can suppress lysoPC-induced expression and secretion of MCP-1 in cultured HUVECs. Therefore, vitamin C is protective against lysoPC-mediated inflammatory insults to the vascular endothelium in vitro.

AB - Background and Purpose: It is well documented that oxidized low-density lipoproteins (LDLs) can stimulate human vascular endothelial cells to produce monocyte chemoattractant protein-1 (MGP-1). Vitamin C is known to be an important antioxidant for vasodilatation. The purpose of this study was to determine whether pretreatment with vitamin C could protect against oxidized-LDL-induced expression of MCP-1 in cultured human umbilical vein endothelial cells (HUVECs). Methods: Cultured HUVECs were used for desired experiments before passage 4. Lysophosphatidylcholine (lysoPC), an oxidized component of LDL, was designated as the stimulator for MCP-1 synthesis from cultured HUVECs. MCP-1 concentrations in the cultured media were determined by enzyme-linked immunosorbent assay. MCP-1 RNA was evaluated by a semi-quantitative reverse transcriptase-polymerase chain reaction. Results: HUVECs secreted MCP-1 within 30 minutes after exposure to 50 μM lysoPC. Compared with samples treated with lysoPC alone, pretreatment with vitamin C in concentrations of 50, 100, 150, and 200 μM, reduced levels of MCP-1 in the culture medium by 44%, 51%, 60%, and 67%, respectively, while levels of MCP-1 mRNA decreased by 15%, 18%, 80%, and 82%, respectively. Conclusions: Our findings imply that pretreatment with vitamin C can suppress lysoPC-induced expression and secretion of MCP-1 in cultured HUVECs. Therefore, vitamin C is protective against lysoPC-mediated inflammatory insults to the vascular endothelium in vitro.

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KW - Cell culture

KW - Lysophosphatidylcholines

KW - Monocyte chemoattractant protein-1

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