Abstract
Inflammation is a defense mechanism against various noxious stimuli. The recruitment of host leukocytes to sites of injury results in increased regional microvascular leakage and reactive oxygen species (ROS) generation. Excessive inflammatory activity not only eliminates offending stimuli but also result in tissue damage, as evidenced in reperfusion injury of the heart. To investigate spatial-temporal evolution of acute inflammation after myocardial reperfusion injury, we monitored microvascular leakage and reactive oxygen species generation with optical mapping technique. Reperfusion injury was performed on isolated blood-perfused rat heart, and it was labeled with dihydroethidium and large molecular weight tetramethylrhodamine conjugated dextran. Tissue was illuminated with a 532 nm laser, and epifluorescence at 580 and 650 nm was collected through 2 separate band pass filters. Our results indicate that 1. Optical mapping of myocardial inflammation is feasible; and 2. Reperfusion injury elicits substantial microvascular leakage and ROS production.
| Original language | English |
|---|---|
| Title of host publication | Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004 |
| Pages | 150 |
| Number of pages | 1 |
| Publication status | Published - 2004 |
| Externally published | Yes |
| Event | Second Asian and Pacific Rim Symposium on Biophotonics, APBP 2004 - Taipei, Taiwan Duration: Dec 15 2004 → Dec 17 2004 |
Other
| Other | Second Asian and Pacific Rim Symposium on Biophotonics, APBP 2004 |
|---|---|
| Country | Taiwan |
| City | Taipei |
| Period | 12/15/04 → 12/17/04 |
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ASJC Scopus subject areas
- Engineering(all)
Cite this
Visualizing inflammatory activity in isolated perfused rat hearts. / Lu, Long Sheng; Liu, Yen Bin; Wu, Chau Chung; Sun, Chia Wei.
Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004. 2004. p. 150.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Visualizing inflammatory activity in isolated perfused rat hearts
AU - Lu, Long Sheng
AU - Liu, Yen Bin
AU - Wu, Chau Chung
AU - Sun, Chia Wei
PY - 2004
Y1 - 2004
N2 - Inflammation is a defense mechanism against various noxious stimuli. The recruitment of host leukocytes to sites of injury results in increased regional microvascular leakage and reactive oxygen species (ROS) generation. Excessive inflammatory activity not only eliminates offending stimuli but also result in tissue damage, as evidenced in reperfusion injury of the heart. To investigate spatial-temporal evolution of acute inflammation after myocardial reperfusion injury, we monitored microvascular leakage and reactive oxygen species generation with optical mapping technique. Reperfusion injury was performed on isolated blood-perfused rat heart, and it was labeled with dihydroethidium and large molecular weight tetramethylrhodamine conjugated dextran. Tissue was illuminated with a 532 nm laser, and epifluorescence at 580 and 650 nm was collected through 2 separate band pass filters. Our results indicate that 1. Optical mapping of myocardial inflammation is feasible; and 2. Reperfusion injury elicits substantial microvascular leakage and ROS production.
AB - Inflammation is a defense mechanism against various noxious stimuli. The recruitment of host leukocytes to sites of injury results in increased regional microvascular leakage and reactive oxygen species (ROS) generation. Excessive inflammatory activity not only eliminates offending stimuli but also result in tissue damage, as evidenced in reperfusion injury of the heart. To investigate spatial-temporal evolution of acute inflammation after myocardial reperfusion injury, we monitored microvascular leakage and reactive oxygen species generation with optical mapping technique. Reperfusion injury was performed on isolated blood-perfused rat heart, and it was labeled with dihydroethidium and large molecular weight tetramethylrhodamine conjugated dextran. Tissue was illuminated with a 532 nm laser, and epifluorescence at 580 and 650 nm was collected through 2 separate band pass filters. Our results indicate that 1. Optical mapping of myocardial inflammation is feasible; and 2. Reperfusion injury elicits substantial microvascular leakage and ROS production.
UR - http://www.scopus.com/inward/record.url?scp=20844441098&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20844441098&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:20844441098
SN - 0780386760
SP - 150
BT - Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004
ER -