Visualizing inflammatory activity in isolated perfused rat hearts

Long Sheng Lu, Yen Bin Liu, Chau Chung Wu, Chia Wei Sun

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Inflammation is a defense mechanism against various noxious stimuli. The recruitment of host leukocytes to sites of injury results in increased regional microvascular leakage and reactive oxygen species (ROS) generation. Excessive inflammatory activity not only eliminates offending stimuli but also result in tissue damage, as evidenced in reperfusion injury of the heart. To investigate spatial-temporal evolution of acute inflammation after myocardial reperfusion injury, we monitored microvascular leakage and reactive oxygen species generation with optical mapping technique. Reperfusion injury was performed on isolated blood-perfused rat heart, and it was labeled with dihydroethidium and large molecular weight tetramethylrhodamine conjugated dextran. Tissue was illuminated with a 532 nm laser, and epifluorescence at 580 and 650 nm was collected through 2 separate band pass filters. Our results indicate that 1. Optical mapping of myocardial inflammation is feasible; and 2. Reperfusion injury elicits substantial microvascular leakage and ROS production.

Original languageEnglish
Title of host publicationSecond Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004
Pages150
Number of pages1
Publication statusPublished - 2004
Externally publishedYes
EventSecond Asian and Pacific Rim Symposium on Biophotonics, APBP 2004 - Taipei, Taiwan
Duration: Dec 15 2004Dec 17 2004

Other

OtherSecond Asian and Pacific Rim Symposium on Biophotonics, APBP 2004
CountryTaiwan
CityTaipei
Period12/15/0412/17/04

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Lu, L. S., Liu, Y. B., Wu, C. C., & Sun, C. W. (2004). Visualizing inflammatory activity in isolated perfused rat hearts. In Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004 (pp. 150)