Visualizing Human Prostate Cancer Cells in Mouse Skeleton Using Bioconjugated Near-infrared Fluorescent Quantum Dots

Chunmeng Shi, Ying Zhu, Zhihui Xie, Weiping Qian, Chia Ling Hsieh, Shuming Nie, Yongping Su, Haiyen E. Zhau, Leland W K Chung

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Objectives: To visualize human prostate cancer cells in mouse bone with bioconjugated near-infrared quantum dot (QD) probes. Near-infrared fluorescent probes using QDs can visualize tumors in deep tissues in vivo. Methods: Human prostate cancer C4-2B xenografts grown in mouse tibia were detected by prostate-specific membrane antigen antibody conjugated with QDs emitting light at the near-infrared range of 800 nm (QD800). Images in culture and in vivo were acquired using the IVIS Imaging System. Results: As few as 5000 cells can be detected subcutaneously when tagged with QD800 conjugate and injected directly into mice. QD800 conjugate injected intravenously in mice harboring C4-2B tumors in tibia detected signals from a minimum of 500 000 cells. The maximal light emission was detected 30 minutes after intravenous injection of QD800 conjugate in mice with established C4-2B tumors. Conclusions: Bioconjugated near-infrared QD probes are highly sensitive molecular imaging tools for human prostate cancer micrometastases in mice.

Original languageEnglish
Pages (from-to)446-451
Number of pages6
JournalUrology
Volume74
Issue number2
DOIs
Publication statusPublished - Aug 2009
Externally publishedYes

Fingerprint

Quantum Dots
Skeleton
Prostatic Neoplasms
Tibia
Light
Neoplasms
Neoplasm Micrometastasis
Molecular Imaging
Fluorescent Dyes
Heterografts
Intravenous Injections
Bone and Bones
Antibodies

ASJC Scopus subject areas

  • Urology

Cite this

Visualizing Human Prostate Cancer Cells in Mouse Skeleton Using Bioconjugated Near-infrared Fluorescent Quantum Dots. / Shi, Chunmeng; Zhu, Ying; Xie, Zhihui; Qian, Weiping; Hsieh, Chia Ling; Nie, Shuming; Su, Yongping; Zhau, Haiyen E.; Chung, Leland W K.

In: Urology, Vol. 74, No. 2, 08.2009, p. 446-451.

Research output: Contribution to journalArticle

Shi, Chunmeng ; Zhu, Ying ; Xie, Zhihui ; Qian, Weiping ; Hsieh, Chia Ling ; Nie, Shuming ; Su, Yongping ; Zhau, Haiyen E. ; Chung, Leland W K. / Visualizing Human Prostate Cancer Cells in Mouse Skeleton Using Bioconjugated Near-infrared Fluorescent Quantum Dots. In: Urology. 2009 ; Vol. 74, No. 2. pp. 446-451.
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abstract = "Objectives: To visualize human prostate cancer cells in mouse bone with bioconjugated near-infrared quantum dot (QD) probes. Near-infrared fluorescent probes using QDs can visualize tumors in deep tissues in vivo. Methods: Human prostate cancer C4-2B xenografts grown in mouse tibia were detected by prostate-specific membrane antigen antibody conjugated with QDs emitting light at the near-infrared range of 800 nm (QD800). Images in culture and in vivo were acquired using the IVIS Imaging System. Results: As few as 5000 cells can be detected subcutaneously when tagged with QD800 conjugate and injected directly into mice. QD800 conjugate injected intravenously in mice harboring C4-2B tumors in tibia detected signals from a minimum of 500 000 cells. The maximal light emission was detected 30 minutes after intravenous injection of QD800 conjugate in mice with established C4-2B tumors. Conclusions: Bioconjugated near-infrared QD probes are highly sensitive molecular imaging tools for human prostate cancer micrometastases in mice.",
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AU - Hsieh, Chia Ling

AU - Nie, Shuming

AU - Su, Yongping

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