Viruses in human tumors. i. hodgkin's disease

Sarah E. Stewart, Elisabeth Z. Mitchell, Jacqueline J. Whang, William R. Dunlop, Theresa Ben, Shigeko Nomura

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A continuous cell culture designated SS5 was established from a lymph node biopsy specimen taken from a male with Hodgkin's disease. The cells grew in suspension as aggregates, in pairs, and singly. During their logarithmic growth the cell doubling time was approximately 24 hours. Chromosome studies were carried out on cells from the patient's bone marrow, the lymph node biopsy before culture, and again after 4 months' culture. On direct examination, the bone marrow and lymph node showed no abnormalities. After 4 months in culture, 89% of the cells examined had 46 chromosomes and were pseudodiploid. There were a low incidence of polyploidy (0.4%) and a low incidence of cells with aberrations (4%). Electron microscope examination showed that the cells carried two kinds of virus-like particles: The herpes-type virus which has been described for cultured Burkitt's tumor cells, and other particles of about the same size with morphology suggestive of a different type of virus. Since the herpes-type virus will not replicate in monolayer cultures, we could separate the two types by inoculating WI38 monolayer cultures with a cell-free concentrate from the SS5 line. Virus replication on passages in the WI38 cells was obvious by specific cellular changes, but only occasional virus-like particles were demonstrable by electron microscopy in the early passages. After 20 passages, abundant virus became evident as extracellular particles. When virus from the WI38 culture was introduced into a human lymphocyte suspension culture, massive virus budding from the cell membrane became evident. The virus ranged from 90–150 mμ, with most about 120 mμ in diameter, and produced a filamentous component 15 mμ in diameter. The filaments were shown to become the nucleoid of the budding virus. The virus was similar immunologically to simian virus SV5.

Original languageEnglish
Pages (from-to)1-14
Number of pages14
JournalJournal of the National Cancer Institute
Volume43
Issue number1
DOIs
Publication statusPublished - Jan 1 1969
Externally publishedYes

Fingerprint

Hodgkin Disease
Viruses
Neoplasms
Virus Release
Lymph Nodes
Virion
Suspensions
Cell Culture Techniques
Chromosomes
Cultured Tumor Cells
Biopsy
Polyploidy
Burkitt Lymphoma
Incidence
Virus Replication
Bone Marrow Cells
Electron Microscopy
Bone Marrow
Cell Membrane
Lymphocytes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Stewart, S. E., Mitchell, E. Z., Whang, J. J., Dunlop, W. R., Ben, T., & Nomura, S. (1969). Viruses in human tumors. i. hodgkin's disease. Journal of the National Cancer Institute, 43(1), 1-14. https://doi.org/10.1093/jnci/43.1.1

Viruses in human tumors. i. hodgkin's disease. / Stewart, Sarah E.; Mitchell, Elisabeth Z.; Whang, Jacqueline J.; Dunlop, William R.; Ben, Theresa; Nomura, Shigeko.

In: Journal of the National Cancer Institute, Vol. 43, No. 1, 01.01.1969, p. 1-14.

Research output: Contribution to journalArticle

Stewart, SE, Mitchell, EZ, Whang, JJ, Dunlop, WR, Ben, T & Nomura, S 1969, 'Viruses in human tumors. i. hodgkin's disease', Journal of the National Cancer Institute, vol. 43, no. 1, pp. 1-14. https://doi.org/10.1093/jnci/43.1.1
Stewart, Sarah E. ; Mitchell, Elisabeth Z. ; Whang, Jacqueline J. ; Dunlop, William R. ; Ben, Theresa ; Nomura, Shigeko. / Viruses in human tumors. i. hodgkin's disease. In: Journal of the National Cancer Institute. 1969 ; Vol. 43, No. 1. pp. 1-14.
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