Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model

Kuan Ying Arthur Huang, Chris Ka Fai Li, Elizabeth Clutterbuck, Cecilia Chui, Tom Wilkinson, Anthony Gilbert, John Oxford, Rob Lambkin-Williams, Tzou Yien Lin, Andrew J. Mcmichael, Xiao Ning Xu

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background. Antibodies play a major role in the protection against influenza virus in human. However, the antibody level is usually short-lived and the cellular mechanisms underlying influenza virus-specific antibody response to acute infection remain unclear.Methods. We studied the kinetics and magnitude of influenza virus-specific B-cell and serum antibody responses in relation to virus replication during the course of influenza infection in healthy adult volunteers who were previously seronegative and experimentally infected with seasonal influenza H1N1 A/Brisbane/59/07 virus.Results. Our data demonstrated a robust expansion of the virus-specific antibody-secreting cells (ASCs) and memory B cells in the peripheral blood, which correlated with both the throat viral load and the duration of viral shedding. The ASC response was obviously detected on day 7 post-infection when the virus was completely cleared from nasal samples, and serum hemagglutination-inhibition antibodies were still undetectable. On day 28 postinfection, influenza virus-specific B cells were further identified from the circulating compartment of isotype-switched B cells.Conclusions. Virus-specific ASCs could be the earliest marker of B-cell response to a new flu virus infection, such as H7N9 in humans.

Original languageEnglish
Pages (from-to)1354-1361
Number of pages8
JournalJournal of Infectious Diseases
Volume209
Issue number9
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Antibody-Producing Cells
Human Influenza
Antibody Formation
Orthomyxoviridae
B-Lymphocytes
Viruses
Virus Diseases
Antibodies
Virus Shedding
Hemagglutination
Virus Replication
Pharynx
Infection
Viral Load
Serum
Nose
Healthy Volunteers

Keywords

  • B cells
  • human challenge model
  • influenza A virus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Huang, K. Y. A., Li, C. K. F., Clutterbuck, E., Chui, C., Wilkinson, T., Gilbert, A., ... Xu, X. N. (2014). Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model. Journal of Infectious Diseases, 209(9), 1354-1361. https://doi.org/10.1093/infdis/jit650

Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model. / Huang, Kuan Ying Arthur; Li, Chris Ka Fai; Clutterbuck, Elizabeth; Chui, Cecilia; Wilkinson, Tom; Gilbert, Anthony; Oxford, John; Lambkin-Williams, Rob; Lin, Tzou Yien; Mcmichael, Andrew J.; Xu, Xiao Ning.

In: Journal of Infectious Diseases, Vol. 209, No. 9, 2014, p. 1354-1361.

Research output: Contribution to journalArticle

Huang, KYA, Li, CKF, Clutterbuck, E, Chui, C, Wilkinson, T, Gilbert, A, Oxford, J, Lambkin-Williams, R, Lin, TY, Mcmichael, AJ & Xu, XN 2014, 'Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model', Journal of Infectious Diseases, vol. 209, no. 9, pp. 1354-1361. https://doi.org/10.1093/infdis/jit650
Huang, Kuan Ying Arthur ; Li, Chris Ka Fai ; Clutterbuck, Elizabeth ; Chui, Cecilia ; Wilkinson, Tom ; Gilbert, Anthony ; Oxford, John ; Lambkin-Williams, Rob ; Lin, Tzou Yien ; Mcmichael, Andrew J. ; Xu, Xiao Ning. / Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model. In: Journal of Infectious Diseases. 2014 ; Vol. 209, No. 9. pp. 1354-1361.
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