Ventilator-induced injury augments interleukin-1β production and neutrophil sequestration in lipopolysaccharide-treated lungs

Shu Min Lin, Horng Chyuan Lin, Kang Yun Lee, Chien Da Huang, Chien Ying Liu, Chun Hua Wang, Han Pin Kuo

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Mechanical ventilators are commonly used to support critically ill patients; however, inappropriate ventilator settings might initiate or augment lung injury. To determine whether a large tidal volume (Vt) augments inflammatory responses and neutrophil sequestration in the lungs of rats receiving intratracheal lipopolysaccharides (LPS). Rats received intratracheal instillation of LPS (0.5 mg/kg) followed by 4 h of mechanical ventilation (MV) at 60 strokes per min with a Vt of 10 mL/kg as control MV, or 30 strokes per min with a Vt of 20 mL/kg of body weight as high-volume MV (HMV). In addition, monoclonal antibodies against rat intercellular adhesion molecule 1 (ICAM-1) or immunoglobulin G (50 mg/kg) were administered 30 min before LPS instillation and MV. Our study demonstrates that HMV enhances pulmonary permeability and induces neutrophil recruitment into the alveolar space and pulmonary edema. Intratracheal instillation of LPS caused marked lung injury, neutrophil recruitment, and production of cytokines and chemokines. Combining LPS instillation and HMV synergistically upregulated interleukin 1β (IL-1β) production and neutrophil sequestration in lung tissues. The ICAM-1 expression in lung tissues was responsible for the synergistic effects of neutrophil sequestration. Synergistic upregulation of IL-1β production and neutrophil sequestration was attenuated by blocking ICAM-1 by neutralizing antibody pretreatment. High Vt MV in LPS-injured lung causes synergistic production of IL-1β and sequestration of neutrophil via ICAM-1-dependent effects.

Original languageEnglish
Pages (from-to)453-460
Number of pages8
JournalShock
Volume28
Issue number4
DOIs
Publication statusPublished - Oct 2007
Externally publishedYes

Fingerprint

Mechanical Ventilators
Interleukin-1
Lipopolysaccharides
Artificial Respiration
Neutrophils
Lung
Wounds and Injuries
Intercellular Adhesion Molecule-1
Neutrophil Infiltration
Lung Injury
Tidal Volume
Pulmonary Edema
Neutralizing Antibodies
Chemokines
Critical Illness
Permeability
Up-Regulation
Immunoglobulin G
Monoclonal Antibodies
Body Weight

Keywords

  • Chemokine
  • Cytokine
  • Lipopolysaccharide
  • Neutrophil
  • Ventilator

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Ventilator-induced injury augments interleukin-1β production and neutrophil sequestration in lipopolysaccharide-treated lungs. / Lin, Shu Min; Lin, Horng Chyuan; Lee, Kang Yun; Huang, Chien Da; Liu, Chien Ying; Wang, Chun Hua; Kuo, Han Pin.

In: Shock, Vol. 28, No. 4, 10.2007, p. 453-460.

Research output: Contribution to journalArticle

Lin, Shu Min ; Lin, Horng Chyuan ; Lee, Kang Yun ; Huang, Chien Da ; Liu, Chien Ying ; Wang, Chun Hua ; Kuo, Han Pin. / Ventilator-induced injury augments interleukin-1β production and neutrophil sequestration in lipopolysaccharide-treated lungs. In: Shock. 2007 ; Vol. 28, No. 4. pp. 453-460.
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