Vasorelaxing and antihypertensive activities of synthesized peptides derived from computer-aided simulation of pepsin hydrolysis of yam dioscorin

Yin Shiou Lin, Yeh Lin Lu, Guei Jane Wang, Hong Jen Liang, Wen Chi Hou

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: We reported that yam dioscorin and its peptic hydrolysates exhibited ACE inhibition and antihypertensive effects on SHRs, however, the active peptides are not really isolated until now. Using ACE inhibitory screenings, two penta-peptides, KTCGY and KRIHF, were selected for ex vivo and in vivo experiments. Results: KTCGY, KRIHF, and captopril were shown to have similar vasodilating effects against phenylephrine (PE)-induced tensions in rat endothelium-dependent thoracic aortic rings, however, KTCGYKTCGY (two-repeated KTCGY) and TCGYTCGY (two-repeated TCGY) were showed endothelium-independent vasodilating effects against PE-induced tensions. KTCGY, KRIHF (10 or 20 mg/kg), and captopril (10 mg/kg) were used to evaluate antihypertensive activity during 24-h after a single oral administration to spontaneously hypertensive rats (SHRs). The KTCGY and KRIHF showed significantly different and reduced the systolic blood pressure of SHRs compared to the blank. Conclusions: These results suggest that KTCGY and KRIHF may contribute important roles in yam dioscorin for regulating blood pressure in vivo.

Original languageEnglish
Article number49
JournalBotanical Studies
Volume55
Issue number1
DOIs
Publication statusPublished - Jun 7 2014

Fingerprint

antihypertensive effect
yams
pepsin
hydrolysis
peptides
phenylephrine
rats
endothelium
Pentas
systolic blood pressure
chest
hydrolysates
oral administration
blood pressure
digestion
screening

Keywords

  • Angiotensin converting enzyme
  • Antihypertensive activity
  • Blood pressure
  • Vasorelaxing
  • Yam dioscorin

ASJC Scopus subject areas

  • Plant Science

Cite this

Vasorelaxing and antihypertensive activities of synthesized peptides derived from computer-aided simulation of pepsin hydrolysis of yam dioscorin. / Lin, Yin Shiou; Lu, Yeh Lin; Wang, Guei Jane; Liang, Hong Jen; Hou, Wen Chi.

In: Botanical Studies, Vol. 55, No. 1, 49, 07.06.2014.

Research output: Contribution to journalArticle

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AU - Hou, Wen Chi

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