Vasorelaxation effects of 2-chloroethanol and chloroacetaldehyde in the isolated rat aortic rings

Yng T. Chen, Dong Zong Hung, Chi Chung Chou, Jaw J. Kang, Yu W. Cheng, Chien-Ming Hu, Jiunn Wang Liao

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

2-Chloroethanol (2-CE) is a commonly used solvent in industry; unfortunately, severe hypotension is one of main toxic signs during intoxication. Calcium ion modulation is considered to be an important role of vasorelaxation. The aim of this study is to evaluate either 2-CE or its main metabolite, chloroacetaldehyde (CAA), possible cause of hypotension, by using isolated rat aortic rings. Results revealed that 2-CE caused a weakly relaxation in the phenylephrine (PE) pre-induced endothelium-intact aortic rings. However, its metabolite, CAA induced vasorelaxation and showed dose dependency in endothelium-intact and -denuded aortic rings. The half inhibitory concentration (IC50) of 2-CE exceeded 50mM; meanwhile, the IC50 values of CAA in the endothelium-intact and -denuded aortic rings were 3.3 and 2.7mM, respectively. The CAA-induced relaxation could be significantly attenuated by adding calcium (CaCl2) and various Ca2+ channel blockers, dantrolene, nifedipine, and NiCl2. Nifedipine presents the most strong inhibition effect among the calcium blockers. In conclusion, it is suggested that the hypotension effect of 2-CE intoxicated cases may be mainly mediated by its metabolite CAA, and calcium channels are partially involved inducing the vasorelaxation.

Original languageEnglish
Pages (from-to)525-531
Number of pages7
JournalJournal of Health Science
Volume55
Issue number4
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Vasodilation
Rats
Metabolites
Hypotension
Endothelium
Nifedipine
Calcium
Inhibitory Concentration 50
Dantrolene
Poisons
Phenylephrine
Calcium Channels
Industry
Modulation
chloroacetaldehyde
Ions

Keywords

  • 2-chloroethanol
  • Calcium channel blocker
  • Chloroacetaldehyde
  • Rat aorta ring
  • Relaxation

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

Vasorelaxation effects of 2-chloroethanol and chloroacetaldehyde in the isolated rat aortic rings. / Chen, Yng T.; Hung, Dong Zong; Chou, Chi Chung; Kang, Jaw J.; Cheng, Yu W.; Hu, Chien-Ming; Liao, Jiunn Wang.

In: Journal of Health Science, Vol. 55, No. 4, 08.2009, p. 525-531.

Research output: Contribution to journalArticle

Chen, Yng T. ; Hung, Dong Zong ; Chou, Chi Chung ; Kang, Jaw J. ; Cheng, Yu W. ; Hu, Chien-Ming ; Liao, Jiunn Wang. / Vasorelaxation effects of 2-chloroethanol and chloroacetaldehyde in the isolated rat aortic rings. In: Journal of Health Science. 2009 ; Vol. 55, No. 4. pp. 525-531.
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AB - 2-Chloroethanol (2-CE) is a commonly used solvent in industry; unfortunately, severe hypotension is one of main toxic signs during intoxication. Calcium ion modulation is considered to be an important role of vasorelaxation. The aim of this study is to evaluate either 2-CE or its main metabolite, chloroacetaldehyde (CAA), possible cause of hypotension, by using isolated rat aortic rings. Results revealed that 2-CE caused a weakly relaxation in the phenylephrine (PE) pre-induced endothelium-intact aortic rings. However, its metabolite, CAA induced vasorelaxation and showed dose dependency in endothelium-intact and -denuded aortic rings. The half inhibitory concentration (IC50) of 2-CE exceeded 50mM; meanwhile, the IC50 values of CAA in the endothelium-intact and -denuded aortic rings were 3.3 and 2.7mM, respectively. The CAA-induced relaxation could be significantly attenuated by adding calcium (CaCl2) and various Ca2+ channel blockers, dantrolene, nifedipine, and NiCl2. Nifedipine presents the most strong inhibition effect among the calcium blockers. In conclusion, it is suggested that the hypotension effect of 2-CE intoxicated cases may be mainly mediated by its metabolite CAA, and calcium channels are partially involved inducing the vasorelaxation.

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