Vascular endothelial growth factor-dependent spinogenesis underlies antidepressant-like effects of enriched environment

Yu Fei Huang, Chih Hao Yang, Chiung Chun Huang, Kuei Sen Hsu

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22 Citations (Scopus)

Abstract

Current antidepressant treatments remain limited by poor efficacy and a slow onset of action. Increasing evidence demonstrates that enriched environment (EE) treatment can promote structural and behavioral plasticity in the brain and dampen stress-induced alterations of neuroplasticity. Here, we have examined whether short term exposure to EE is able to produce antidepressant-like effects. Our results show that housing adult mice in an EE cage for 7 days led to antidepressant-like behavioral profiles and a significant increase in the number of dendritic spines in hippocampal CA1 pyramidal neurons. These EE-induced antidepressant-like effects are primarily attributed to increased vascular endothelial growth factor (VEGF) expression through a hypoxia-inducible factor-1α (HIF-1α)-mediated transcriptional mechanism. Blockade of HIF-1α synthesis by lentiviral infection with HIF-1α small hairpin RNAs completely blocked the increase in expression of VEGF and the antidepressant-like effects induced by EE. Moreover, no significant antidepressant-like effects were observed with EE treatment in VEGF receptor 2 (Flk-1) knock-out mice. The increase in HIF-1αexpression in the hippocampus induced by EE was associated with a decrease in endogenous levels of microRNA-107 (miR-107). Overexpression of miR-107 in the hippocampus completely blocked EE-induced HIF-1α expression and the antidepressant-like effects. These results support a model in which the down-regulation of miR-107, acting through HIF-1α, mediates VEGF-dependent spinogenesis to underlie the EE-induced antidepressant-like effects.

Original languageEnglish
Pages (from-to)40938-40955
Number of pages18
JournalJournal of Biological Chemistry
Volume287
Issue number49
DOIs
Publication statusPublished - Nov 30 2012
Externally publishedYes

Fingerprint

Vascular Endothelial Growth Factor A
Antidepressive Agents
Hypoxia-Inducible Factor 1
MicroRNAs
Hippocampus
Vascular Endothelial Growth Factor Receptor-2
Dendritic Spines
Neuronal Plasticity
Pyramidal Cells
Small Interfering RNA
Neurons
Plasticity
Knockout Mice
Brain
Down-Regulation
Infection

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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Vascular endothelial growth factor-dependent spinogenesis underlies antidepressant-like effects of enriched environment. / Huang, Yu Fei; Yang, Chih Hao; Huang, Chiung Chun; Hsu, Kuei Sen.

In: Journal of Biological Chemistry, Vol. 287, No. 49, 30.11.2012, p. 40938-40955.

Research output: Contribution to journalArticle

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