Vancomycin-resistant enterococci in a tertiary teaching hospital in Taiwan

Yu Chia Hsieh, Tsong Yih Ou, Sing On Teng, Wuan Chan Lee, Yi Chun Lin, Jann Tay Wang, Shan Chwen Chang, Wen Sen Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background and purpose: In 2007, an outbreak of vancomycin-resistant enterococci (VRE) occurred at Taipei Medical University, Wan Fang Hospital, Taipei, Taiwan. The aim of this study was to characterize the mechanism of glycopeptide resistance and to investigate the genetic relatedness among isolates of VRE. Methods: Between May and October 2007, bacterial isolates from 16 patients identified as colonized or infected with VRE were collected. Polymerase chain reaction and pulsed-field gel electrophoresis (PFGE) were used to determine resistant genes and molecular typing. Results: All 16 isolates of VRE presented with the VanA phenotype with the vanA gene except for 1 isolate of Enterococcus faecalis, which had the VanB phenotype with the vanA gene. PFGE analysis revealed a major clone containing 12 isolates, and 4 other distinct clones containing 1 to 2 isolates each. Five patients had VRE colonized in their gastrointestinal tract, the genotype of which was the same as the clinical isolates. Fourteen isolates (87.5%) had the esp gene. Conclusions: An outbreak of VRE was caused by the simultaneous existence of monoclonal and polyclonal spread. Rigorous infection control, active surveillance, and decreasing pressure of antibiotic use are important for controlling the emergence of VRE.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalJournal of Microbiology, Immunology and Infection
Volume42
Issue number1
Publication statusPublished - Feb 2009

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Taiwan
Tertiary Care Centers
Teaching Hospitals
Pulsed Field Gel Electrophoresis
Genes
Disease Outbreaks
Clone Cells
Phenotype
Molecular Typing
Glycopeptides
Enterococcus faecalis
Infection Control
Vancomycin-Resistant Enterococci
Gastrointestinal Tract
Genotype
Anti-Bacterial Agents
Pressure
Polymerase Chain Reaction

Keywords

  • Bacterial
  • Drug resistance
  • Electrophoresis
  • Enterococcus
  • Gel
  • Genes
  • Pulsed-field
  • Vancomycin resistance

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Infectious Diseases

Cite this

Vancomycin-resistant enterococci in a tertiary teaching hospital in Taiwan. / Hsieh, Yu Chia; Ou, Tsong Yih; Teng, Sing On; Lee, Wuan Chan; Lin, Yi Chun; Wang, Jann Tay; Chang, Shan Chwen; Lee, Wen Sen.

In: Journal of Microbiology, Immunology and Infection, Vol. 42, No. 1, 02.2009, p. 63-68.

Research output: Contribution to journalArticle

Hsieh, YC, Ou, TY, Teng, SO, Lee, WC, Lin, YC, Wang, JT, Chang, SC & Lee, WS 2009, 'Vancomycin-resistant enterococci in a tertiary teaching hospital in Taiwan', Journal of Microbiology, Immunology and Infection, vol. 42, no. 1, pp. 63-68.
Hsieh, Yu Chia ; Ou, Tsong Yih ; Teng, Sing On ; Lee, Wuan Chan ; Lin, Yi Chun ; Wang, Jann Tay ; Chang, Shan Chwen ; Lee, Wen Sen. / Vancomycin-resistant enterococci in a tertiary teaching hospital in Taiwan. In: Journal of Microbiology, Immunology and Infection. 2009 ; Vol. 42, No. 1. pp. 63-68.
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