UVB Irradiation Regulates ERK1/2- and p53-Dependent Thrombomodulin Expression in Human Keratinocytes

Huey Chun Huang, Tsong Min Chang, Yu Jia Chang, Hsin Yun Wen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Thrombomodulin (TM) is highly expressed in endothelial cells and acts as a natural anticoagulation factor to maintain circulation homeostasis. TM is an interesting molecule with many physiological functions, including anti-inflammation, anti-thrombosis, and carcinogenesis inhibition. TM can also be detected on the spinous layer of epidermal keratinocytes. However, the role of epidermal TM is still under investigation. In this study, we investigated keratinocyte TM expression and regulation in response to sub-cytotoxic ultraviolet B (UVB) irradiation. Oxidative stress was assessed with DCF and the results revealed that UVB irradiation significantly and dose-dependently augmented reactive oxygen species (ROS) production in HaCaT cells. In addition, low-dose UVB irradiation decreased TM mRNA and protein levels. Blocking ROS production and ERK activation prevented UVB-induced TM down-regulation. The nuclear p53 accumulation and TM promoter binding was observed within 3 h after UVB exposure. Small interfering RNA-mediated p53 knockdown disrupted the UVB-mediated TM protein down-regulation. Our study demonstrates that UVB irradiation results in ROS accumulation and ERK activation, which causes the nuclear p53 accumulation and TM promoter binding to inhibit TM expression. This study provides novel evidence demonstrating that p53 serves as a key regulator of keratinocyte TM expression.

Original languageEnglish
Article numbere67632
JournalPLoS One
Volume8
Issue number7
DOIs
Publication statusPublished - Jul 2 2013

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Thrombomodulin
keratinocytes
Keratinocytes
ultraviolet radiation
irradiation
Irradiation
reactive oxygen species
Reactive Oxygen Species
thrombosis
Dosimetry
dosage
small interfering RNA
carcinogenesis
Down-Regulation
endothelial cells
Chemical activation
homeostasis
oxidative stress
proteins
inflammation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

UVB Irradiation Regulates ERK1/2- and p53-Dependent Thrombomodulin Expression in Human Keratinocytes. / Huang, Huey Chun; Chang, Tsong Min; Chang, Yu Jia; Wen, Hsin Yun.

In: PLoS One, Vol. 8, No. 7, e67632, 02.07.2013.

Research output: Contribution to journalArticle

Huang, Huey Chun ; Chang, Tsong Min ; Chang, Yu Jia ; Wen, Hsin Yun. / UVB Irradiation Regulates ERK1/2- and p53-Dependent Thrombomodulin Expression in Human Keratinocytes. In: PLoS One. 2013 ; Vol. 8, No. 7.
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