Utilization of IκB-EGFP Chimeric Gene as an Indicator to Identify Microbial Metabolites with NF-κB Inhibitor Activity

Yu Ling Lin, Yen Shun Chen, Jui Hung Hsieh, Ching Min Lin, Hsin Yi Wu, Chen Si Lin, Rea Min Chu, Kuang Wen Liao, Yuan Hsun Hsu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

NF-κB regulates several important expressions, such as cytokine release, anti-apoptosis, adhesion molecule expression, and cell cycle processing. Several NF-κB inhibitors have been discovered as an anti-tumor or antiinflammatory drug. The activity of NF-κB transcription factor is negatively regulated by IκB binding. In this study, IκB assay system was established and IκB-EGFP fusion protein was used as an indicator to monitor the effects of substances on the IκB degradation. The results indicated that the chosen hydroquinone could inhibit the IκB degradation and cause the cell de-attachment from the bottom of culture plate. In addition, this system could also monitor the IκB degradation of microbial metabolite of natural mixtures of propolis. Thus, the IκB assay system may be a good system for drug discovery related to microbial metabolite.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalBiological Procedures Online
Volume12
Issue number1
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Propolis
Drug Discovery
Metabolites
Cell Cycle
Anti-Inflammatory Agents
Transcription Factors
Genes
Apoptosis
Cytokines
Degradation
Assays
Pharmaceutical Preparations
Neoplasms
Tumors
Fusion reactions
Adhesion
Cells
Molecules
Processing
3'-(1-butylphosphoryl)adenosine

Keywords

  • Antioxidant
  • EGFP. Hydroquinone
  • IκB
  • Microbial metabolite
  • Propolis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Utilization of IκB-EGFP Chimeric Gene as an Indicator to Identify Microbial Metabolites with NF-κB Inhibitor Activity. / Lin, Yu Ling; Chen, Yen Shun; Hsieh, Jui Hung; Lin, Ching Min; Wu, Hsin Yi; Lin, Chen Si; Chu, Rea Min; Liao, Kuang Wen; Hsu, Yuan Hsun.

In: Biological Procedures Online, Vol. 12, No. 1, 12.2010, p. 131-138.

Research output: Contribution to journalArticle

Lin, Yu Ling ; Chen, Yen Shun ; Hsieh, Jui Hung ; Lin, Ching Min ; Wu, Hsin Yi ; Lin, Chen Si ; Chu, Rea Min ; Liao, Kuang Wen ; Hsu, Yuan Hsun. / Utilization of IκB-EGFP Chimeric Gene as an Indicator to Identify Microbial Metabolites with NF-κB Inhibitor Activity. In: Biological Procedures Online. 2010 ; Vol. 12, No. 1. pp. 131-138.
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