USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy

Yi Chang Wang, Yu Syuan Wu, Chia Yang Hung, Shao An Wang, Ming Jer Young, Tsung I. Hsu, Jan Jong Hung

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have previously demonstrated that USP24 is involved in cancer progression. Here, we found that USP24 expression is upregulated in M2 macrophages and lung cancer cells. Conditioned medium from USP24-knockdown M2 macrophages decreases the migratory and chemotactic activity of lung cancer cells and the angiogenic properties of human microvascular endothelial cell 1 (HMEC-1). IL-6 expression is significantly decreased in USP24-knockdown M2 macrophages and lung cancer cells, and IL-6-replenished conditioned medium restores the migratory, chemotactic and angiogenetic properties of the cells. USP24 stabilizes p300 and β-TrCP to increase the levels of histone-3 acetylation and NF-κB, and decreases the levels of DNMT1 and IκB, thereby increasing IL-6 transcription in M2 macrophages and lung cancer cells, results in cancer malignancy finally. IL-6 has previously been a target for cancer drug development. Here, we provide direct evidence to support that USP24 promotes IL-6 expression, which might be beneficial for cancer therapy.

Original languageEnglish
Article number3996
JournalNature Communications
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Tumor Microenvironment
Macrophages
Tumors
Interleukin-6
tumors
cancer
Lung Neoplasms
macrophages
Cells
lungs
Neoplasms
Conditioned Culture Medium
Acetylation
Endothelial cells
Transcription
Histones
acetylation
Endothelial Cells
progressions
therapy

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy. / Wang, Yi Chang; Wu, Yu Syuan; Hung, Chia Yang; Wang, Shao An; Young, Ming Jer; Hsu, Tsung I.; Hung, Jan Jong.

In: Nature Communications, Vol. 9, No. 1, 3996, 01.12.2018.

Research output: Contribution to journalArticle

Wang, Yi Chang ; Wu, Yu Syuan ; Hung, Chia Yang ; Wang, Shao An ; Young, Ming Jer ; Hsu, Tsung I. ; Hung, Jan Jong. / USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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