Using sweeping-micellar electrokinetic chromatography to determine melamine in food

Wen Chi Wu, I. Lin Tsai, Shao Wen Sun, Ching Hua Kuo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

This study developed a sweeping-micellar electrokinetic chromatography (sweeping-MEKC) method for fast screening of melamine in food. In order to optimise parameters with multiple responses in parallel, face-centred cube central composite design (FCD) was employed to search for the optimum conditions of the sweeping-MEKC. The optimum background electrolyte was composed of 45 mM phosphoric acid and 175 mM sodium dodecyl-sulfate with the addition of 15% (v/v) methanol. Under such conditions, melamine content could be determined within 13 min with the limit of detection (S/N = 3) at 5 ng/mL. The developed method was validated by analysing melamine-spiked milk, gluten, chicken feed, and cookies. The proposed method was applied to determine melamine in different food products, with recoveries between 92.9 ± 3.3% and 108.4 ± 1.7%. The present study shows that the developed sweeping-MEKC method is sensitive, accurate, and efficient for fast screening of melamine in food.

Original languageEnglish
Pages (from-to)783-789
Number of pages7
JournalFood Chemistry
Volume128
Issue number3
DOIs
Publication statusPublished - Oct 1 2011
Externally publishedYes

Fingerprint

melamine
Chromatography
chromatography
Food
Screening
screening
cookies
phosphoric acid
Glutens
sodium dodecyl sulfate
gluten
methodology
Sodium Dodecyl Sulfate
Electrolytes
electrolytes
Methanol
Limit of Detection
Chickens
Milk
detection limit

Keywords

  • Face-centred cube central composite design (FCD)
  • Food
  • Melamine
  • Sweeping-MEKC

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Using sweeping-micellar electrokinetic chromatography to determine melamine in food. / Wu, Wen Chi; Tsai, I. Lin; Sun, Shao Wen; Kuo, Ching Hua.

In: Food Chemistry, Vol. 128, No. 3, 01.10.2011, p. 783-789.

Research output: Contribution to journalArticle

Wu, Wen Chi ; Tsai, I. Lin ; Sun, Shao Wen ; Kuo, Ching Hua. / Using sweeping-micellar electrokinetic chromatography to determine melamine in food. In: Food Chemistry. 2011 ; Vol. 128, No. 3. pp. 783-789.
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