Using siRNA technique to generate transgenic animals with spatiotemporal and conditional gene knockdown

Hung Shu Chang, Ching Hui Lin, Yu Chi Chen, Winston C Y Yu

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Based on the RNAi technique, we have developed a new approach that generates transgenic animals capable of mimicking human genetic diseases. The new system is a combination of siRNA with Cre-loxP and tetracycline-on. It has the characteristics of being stable, inheritable, and inducible, with the siRNA able to be transcribed tissue specifically. To support the ability of this new method to generate a model for a disease, we created an ABCA1-deficient mouse line that mimics Tangier disease under controlled conditions. Thus, it should now be possible to rapidly establish human genetic diseases as a whole animal model without the use of embryonic stem cell and gene targeting. This system also provides a tool for pathological and pharmacological studies of aspects peculiar to particular human genetic diseases.

Original languageEnglish
Pages (from-to)1535-1541
Number of pages7
JournalAmerican Journal of Pathology
Volume165
Issue number5
Publication statusPublished - Nov 2004
Externally publishedYes

Fingerprint

Gene Knockdown Techniques
Inborn Genetic Diseases
Genetically Modified Animals
Medical Genetics
Small Interfering RNA
Tangier Disease
Gene Targeting
Embryonic Stem Cells
RNA Interference
Tetracycline
Animal Models
Pharmacology

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Using siRNA technique to generate transgenic animals with spatiotemporal and conditional gene knockdown. / Chang, Hung Shu; Lin, Ching Hui; Chen, Yu Chi; Yu, Winston C Y.

In: American Journal of Pathology, Vol. 165, No. 5, 11.2004, p. 1535-1541.

Research output: Contribution to journalArticle

Chang, Hung Shu ; Lin, Ching Hui ; Chen, Yu Chi ; Yu, Winston C Y. / Using siRNA technique to generate transgenic animals with spatiotemporal and conditional gene knockdown. In: American Journal of Pathology. 2004 ; Vol. 165, No. 5. pp. 1535-1541.
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