Using siRNA technique to generate transgenic animals with spatiotemporal and conditional gene knockdown

Hung Shu Chang, Ching Hui Lin, Yu Chi Chen, Winston C Y Yu

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Based on the RNAi technique, we have developed a new approach that generates transgenic animals capable of mimicking human genetic diseases. The new system is a combination of siRNA with Cre-loxP and tetracycline-on. It has the characteristics of being stable, inheritable, and inducible, with the siRNA able to be transcribed tissue specifically. To support the ability of this new method to generate a model for a disease, we created an ABCA1-deficient mouse line that mimics Tangier disease under controlled conditions. Thus, it should now be possible to rapidly establish human genetic diseases as a whole animal model without the use of embryonic stem cell and gene targeting. This system also provides a tool for pathological and pharmacological studies of aspects peculiar to particular human genetic diseases.

Original languageEnglish
Pages (from-to)1535-1541
Number of pages7
JournalAmerican Journal of Pathology
Volume165
Issue number5
Publication statusPublished - Nov 2004
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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