Using Gelatin Scaffold With Coated Basic Fibroblast Growth Factor as a Transfer System for Transplantation of Human Neural Stem Cells

Y. W. Chen, S. H. Chiou, Tai-Tong Wong, H. H. Ku, H. T. Lin, C. F. Chung, S. H. Yen, C. L. Kao

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Gelatin scaffolds for ex vivo cell cultures are a promising development. These scaffolds can be used as three-dimensional skeletons for cell attachment and culture before transplantation. In this study, we isolated and cultivated neural stem cells from human brain tissues in serum-free medium (DMEM+F12 nutrient). Better neuron growth was observed using the tetrazolium assay (MTT) in the group when basic fibroblast growth factor (bFGF) was coated on the gelatin polymer scaffold. Further development of this nontoxic system may help the future development of transplantation of human neural stem cells.

Original languageEnglish
Pages (from-to)1616-1617
Number of pages2
JournalTransplantation Proceedings
Volume38
Issue number5
DOIs
Publication statusPublished - Jun 1 2006
Externally publishedYes

Fingerprint

Neural Stem Cells
Fibroblast Growth Factor 2
Gelatin
Cell Culture Techniques
Transplantation
Serum-Free Culture Media
Human Development
Skeleton
Polymers
Neurons
Food
Brain
Growth
thiazolyl blue

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Using Gelatin Scaffold With Coated Basic Fibroblast Growth Factor as a Transfer System for Transplantation of Human Neural Stem Cells. / Chen, Y. W.; Chiou, S. H.; Wong, Tai-Tong; Ku, H. H.; Lin, H. T.; Chung, C. F.; Yen, S. H.; Kao, C. L.

In: Transplantation Proceedings, Vol. 38, No. 5, 01.06.2006, p. 1616-1617.

Research output: Contribution to journalArticle

Chen, Y. W. ; Chiou, S. H. ; Wong, Tai-Tong ; Ku, H. H. ; Lin, H. T. ; Chung, C. F. ; Yen, S. H. ; Kao, C. L. / Using Gelatin Scaffold With Coated Basic Fibroblast Growth Factor as a Transfer System for Transplantation of Human Neural Stem Cells. In: Transplantation Proceedings. 2006 ; Vol. 38, No. 5. pp. 1616-1617.
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