14 Citations (Scopus)

Abstract

Aim of the study: This study examined the modulating effects of Clematis chinensis Osbeck (Ranunculaeae) on pro-inflammatory and degradative mediators associated with inflammatory arthritis. Materials and methods: Primary human chondrocytes (PHC) were stimulated with IL-1β or lipopolysaccharide (LPS) to induce the enhanced release of prostaglandin E2 (PGE2), metalloproteinase (MMP-3 and -13), and cyclooxygenase-2 (COX-2) protein expression. The 18F-FDG microPET imaging system was used to evaluate the anti-arthritic effects of Clematis chinensis in vivo. Results: The acetone extracted Clematis chinensis (CC6) contained the most total saponins compared to other solvent's extracts and showed significant and dose-dependent inhibitory effects on PGE2, MMP-3, -13, and COX-2 productions by LPS-stimulated PHC. Furthermore, CC6 also exerted inhibitory effects on 2-18F- fluoro-2-deoxy-d-glucose (18F-FDG) uptake when assessed by positron emission tomography (PET) uptake in the joints and serum PGE2 of rabbits with knee joints injected with LPS. Conclusion: The results suggest the significant chondroprotective effects of Clematis chinensis are through its anti-inflammatory and MMPs inhibitory abilities. Meanwhile, we established a new analysis method to evaluate the Chinese herbal anti-arthritic effects.

Original languageEnglish
Pages (from-to)511-517
Number of pages7
JournalJournal of Ethnopharmacology
Volume136
Issue number3
DOIs
Publication statusPublished - Jul 14 2011

Fingerprint

Clematis
Arthritis
Dinoprostone
Lipopolysaccharides
Glucose
Cyclooxygenase 2
Chondrocytes
Matrix Metalloproteinases
Matrix Metalloproteinase 13
Matrix Metalloproteinase 3
Saponins
Knee Joint
Acetone
Interleukin-1
Positron-Emission Tomography
Anti-Inflammatory Agents
Joints
Rabbits
Serum
Proteins

Keywords

  • Arthritis
  • Chondrocytes
  • Clematis chinensis Osbeck
  • Lipopolysaccharide
  • Metalloproteinase
  • Positron emission tomography
  • Prostaglandin E2

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

@article{d13b3ed91bce4f8387bf5d107a9f169c,
title = "Using 18F-FDG microPET imaging to measure the inhibitory effects of Clematis chinensis Osbeck on the pro-inflammatory and degradative mediators associated with inflammatory arthritis",
abstract = "Aim of the study: This study examined the modulating effects of Clematis chinensis Osbeck (Ranunculaeae) on pro-inflammatory and degradative mediators associated with inflammatory arthritis. Materials and methods: Primary human chondrocytes (PHC) were stimulated with IL-1β or lipopolysaccharide (LPS) to induce the enhanced release of prostaglandin E2 (PGE2), metalloproteinase (MMP-3 and -13), and cyclooxygenase-2 (COX-2) protein expression. The 18F-FDG microPET imaging system was used to evaluate the anti-arthritic effects of Clematis chinensis in vivo. Results: The acetone extracted Clematis chinensis (CC6) contained the most total saponins compared to other solvent's extracts and showed significant and dose-dependent inhibitory effects on PGE2, MMP-3, -13, and COX-2 productions by LPS-stimulated PHC. Furthermore, CC6 also exerted inhibitory effects on 2-18F- fluoro-2-deoxy-d-glucose (18F-FDG) uptake when assessed by positron emission tomography (PET) uptake in the joints and serum PGE2 of rabbits with knee joints injected with LPS. Conclusion: The results suggest the significant chondroprotective effects of Clematis chinensis are through its anti-inflammatory and MMPs inhibitory abilities. Meanwhile, we established a new analysis method to evaluate the Chinese herbal anti-arthritic effects.",
keywords = "Arthritis, Chondrocytes, Clematis chinensis Osbeck, Lipopolysaccharide, Metalloproteinase, Positron emission tomography, Prostaglandin E2",
author = "Ming-Shium Hsieh and Wang, {Kun Teng} and Tseng, {Sung Hui} and Lee, {Chia Jung} and Chi-Hsien Chen and Wang, {Ching Chiung}",
year = "2011",
month = "7",
day = "14",
doi = "10.1016/j.jep.2010.06.042",
language = "English",
volume = "136",
pages = "511--517",
journal = "Journal of Ethnopharmacology",
issn = "0378-8741",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Using 18F-FDG microPET imaging to measure the inhibitory effects of Clematis chinensis Osbeck on the pro-inflammatory and degradative mediators associated with inflammatory arthritis

AU - Hsieh, Ming-Shium

AU - Wang, Kun Teng

AU - Tseng, Sung Hui

AU - Lee, Chia Jung

AU - Chen, Chi-Hsien

AU - Wang, Ching Chiung

PY - 2011/7/14

Y1 - 2011/7/14

N2 - Aim of the study: This study examined the modulating effects of Clematis chinensis Osbeck (Ranunculaeae) on pro-inflammatory and degradative mediators associated with inflammatory arthritis. Materials and methods: Primary human chondrocytes (PHC) were stimulated with IL-1β or lipopolysaccharide (LPS) to induce the enhanced release of prostaglandin E2 (PGE2), metalloproteinase (MMP-3 and -13), and cyclooxygenase-2 (COX-2) protein expression. The 18F-FDG microPET imaging system was used to evaluate the anti-arthritic effects of Clematis chinensis in vivo. Results: The acetone extracted Clematis chinensis (CC6) contained the most total saponins compared to other solvent's extracts and showed significant and dose-dependent inhibitory effects on PGE2, MMP-3, -13, and COX-2 productions by LPS-stimulated PHC. Furthermore, CC6 also exerted inhibitory effects on 2-18F- fluoro-2-deoxy-d-glucose (18F-FDG) uptake when assessed by positron emission tomography (PET) uptake in the joints and serum PGE2 of rabbits with knee joints injected with LPS. Conclusion: The results suggest the significant chondroprotective effects of Clematis chinensis are through its anti-inflammatory and MMPs inhibitory abilities. Meanwhile, we established a new analysis method to evaluate the Chinese herbal anti-arthritic effects.

AB - Aim of the study: This study examined the modulating effects of Clematis chinensis Osbeck (Ranunculaeae) on pro-inflammatory and degradative mediators associated with inflammatory arthritis. Materials and methods: Primary human chondrocytes (PHC) were stimulated with IL-1β or lipopolysaccharide (LPS) to induce the enhanced release of prostaglandin E2 (PGE2), metalloproteinase (MMP-3 and -13), and cyclooxygenase-2 (COX-2) protein expression. The 18F-FDG microPET imaging system was used to evaluate the anti-arthritic effects of Clematis chinensis in vivo. Results: The acetone extracted Clematis chinensis (CC6) contained the most total saponins compared to other solvent's extracts and showed significant and dose-dependent inhibitory effects on PGE2, MMP-3, -13, and COX-2 productions by LPS-stimulated PHC. Furthermore, CC6 also exerted inhibitory effects on 2-18F- fluoro-2-deoxy-d-glucose (18F-FDG) uptake when assessed by positron emission tomography (PET) uptake in the joints and serum PGE2 of rabbits with knee joints injected with LPS. Conclusion: The results suggest the significant chondroprotective effects of Clematis chinensis are through its anti-inflammatory and MMPs inhibitory abilities. Meanwhile, we established a new analysis method to evaluate the Chinese herbal anti-arthritic effects.

KW - Arthritis

KW - Chondrocytes

KW - Clematis chinensis Osbeck

KW - Lipopolysaccharide

KW - Metalloproteinase

KW - Positron emission tomography

KW - Prostaglandin E2

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U2 - 10.1016/j.jep.2010.06.042

DO - 10.1016/j.jep.2010.06.042

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C2 - 20599489

AN - SCOPUS:79955003351

VL - 136

SP - 511

EP - 517

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

SN - 0378-8741

IS - 3

ER -