Usefulness of human leucocyte antigen-B27 subtypes in predicting ankylosing spondylitis: Taiwan experience

T. Y. Hou, H. C. Chen, C. H. Chen, D. M. Chang, F. C. Liu, J. H. Lai

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Background: Genetic factors are clearly attributed to the susceptibility of ankylosing spondylitis (AS). The human leucocyte antigen (HLA)-B27 proved to be the very useful marker for diagnosing AS. The aim of this study was to determine the prevalence of HLA-B27 subtypes in Taiwan and to investigate whether these subtypes may be of help in predicting the diagnosis of AS. Methods: A total of 314 patients with AS and a control group of 71 subjects positive for HLA-B27 detected by flow cytometry analysis were recruited for the study. HLA-B27 subtypes were confirmed by the polymerase chain reaction-sequence-specific primers and sequence-specific oligonucleotide probing. Results: Four B27 alleles were identified: B*2704, B*2705, B*2706 and B*2707. HLA-B*2704 was the predominant allele. There were significant differences in the distribution of HLA-B27 subtypes between patients with AS and controls. Five of them who were homozygous for the B*2704 allele were solely found in AS group but not in controls. Statistical analysis showed that B*2704 was positively associated with AS, which suggested an increased possibility of having AS. Other HLA-B27 subtypes showed no strong correlation with AS. Conclusion: In the Taiwanese population, susceptibility to AS was determined by the presence of HLA-B*2704. Although B*2706 was reported to have a negative association with AS in Taiwanese, Thai and Chinese Singaporean populations, we report, in our study, two AS patients with B*2706 (0.6%). Disease heterogeneity suggests that other than genetic background, many pathogenic factors could be associated with AS. This may need to be investigated with a larger group of patients with AS and controls.

Original languageEnglish
Pages (from-to)749-752
Number of pages4
JournalInternal Medicine Journal
Volume37
Issue number11
DOIs
Publication statusPublished - Nov 1 2007
Externally publishedYes

Fingerprint

Ankylosing Spondylitis
HLA Antigens
Taiwan
Alleles
Oligonucleotides
Population
Flow Cytometry

Keywords

  • Ankylosing spondylitis
  • HLA-B27 subtype

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Usefulness of human leucocyte antigen-B27 subtypes in predicting ankylosing spondylitis : Taiwan experience. / Hou, T. Y.; Chen, H. C.; Chen, C. H.; Chang, D. M.; Liu, F. C.; Lai, J. H.

In: Internal Medicine Journal, Vol. 37, No. 11, 01.11.2007, p. 749-752.

Research output: Contribution to journalArticle

Hou, T. Y. ; Chen, H. C. ; Chen, C. H. ; Chang, D. M. ; Liu, F. C. ; Lai, J. H. / Usefulness of human leucocyte antigen-B27 subtypes in predicting ankylosing spondylitis : Taiwan experience. In: Internal Medicine Journal. 2007 ; Vol. 37, No. 11. pp. 749-752.
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abstract = "Background: Genetic factors are clearly attributed to the susceptibility of ankylosing spondylitis (AS). The human leucocyte antigen (HLA)-B27 proved to be the very useful marker for diagnosing AS. The aim of this study was to determine the prevalence of HLA-B27 subtypes in Taiwan and to investigate whether these subtypes may be of help in predicting the diagnosis of AS. Methods: A total of 314 patients with AS and a control group of 71 subjects positive for HLA-B27 detected by flow cytometry analysis were recruited for the study. HLA-B27 subtypes were confirmed by the polymerase chain reaction-sequence-specific primers and sequence-specific oligonucleotide probing. Results: Four B27 alleles were identified: B*2704, B*2705, B*2706 and B*2707. HLA-B*2704 was the predominant allele. There were significant differences in the distribution of HLA-B27 subtypes between patients with AS and controls. Five of them who were homozygous for the B*2704 allele were solely found in AS group but not in controls. Statistical analysis showed that B*2704 was positively associated with AS, which suggested an increased possibility of having AS. Other HLA-B27 subtypes showed no strong correlation with AS. Conclusion: In the Taiwanese population, susceptibility to AS was determined by the presence of HLA-B*2704. Although B*2706 was reported to have a negative association with AS in Taiwanese, Thai and Chinese Singaporean populations, we report, in our study, two AS patients with B*2706 (0.6{\%}). Disease heterogeneity suggests that other than genetic background, many pathogenic factors could be associated with AS. This may need to be investigated with a larger group of patients with AS and controls.",
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